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Aconitase 1 Antibody (22C10), Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NBP3-26529

Recombinant monoclonal antibody expressed in HEK293F cells
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NBP3-26529-50ul
NBP3-26529-100ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 22C10

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Aconitase 1 Antibody (22C10)

Immunogen

A synthesized peptide derived from Human Aconitase 1 [UniProt P21399]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Aconitase 1 Antibody (22C10)

Aconitase 1 Antibody (22C10)

Western Blot: Aconitase 1 Antibody (22C10) [NBP3-26529] -

Western Blot: Aconitase 1 Antibody (22C10) [NBP3-26529] - Positive Western Blot detected in: Hela whole cell lysate.
All lanes: Aconitase 1 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 100 kDa
Observed band size: 95-130 kDa
Aconitase 1 Antibody (22C10)

Immunocytochemistry/Immunofluorescence: Aconitase 1 Antibody (22C10) [NBP3-26529] -

Immunocytochemistry/Immunofluorescence: Aconitase 1 Antibody (22C10) [NBP3-26529] - Staining of HepG2 cell with Aconitase 1 Antibody (22C10) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 511-conjugated Goat Anti-Rabbit IgG (H+L).
Aconitase 1 Antibody (22C10)

Flow Cytometry: Aconitase 1 Antibody (22C10) [NBP3-26529] -

Flow Cytometry: Aconitase 1 Antibody (22C10) [NBP3-26529] - Overlay Peak curve showing HepG2 cells stained with Aconitase 1 Antibody (22C10) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

Applications for Aconitase 1 Antibody (22C10)

Application
Recommended Usage

Flow Cytometry

1:50-1:200

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Aconitase 1

Aconitase 1, also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein which binds to iron-responsive elements (IREs). IREs are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. The iron-induced binding to the IRE results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degrading transferrin receptor mRNA. Thus, IREB1 plays a central role in cellular iron homeostasis. It was also shown to have aconitase activity, and hence grouped with the aconitase family of enzymes.

Alternate Names

Aconitase, aconitase 1, soluble, aconitate hydratase, Citrate hydro-lyase, cytoplasmic aconitate hydratase, EC 4.2.1, EC 4.2.1.3, Ferritin repressor protein, IREB1IREBP1, IREBP, IRE-BP 1, Iron regulatory protein 1, iron-responsive element binding protein 1, Iron-responsive element-binding protein 1, IRP1ACONS

Gene Symbol

ACO1

Additional Aconitase 1 Products

Product Documents for Aconitase 1 Antibody (22C10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Aconitase 1 Antibody (22C10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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