Acetyl-Lysine Antibody (RM101) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25979

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25979
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Key Product Details

Validated by

Biological Validation

Species Reactivity

All Species

Applications

Chromatin Immunoprecipitation (ChIP), ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM101

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Acetyl-Lysine Antibodies »

Product Specifications

Immunogen

Acetyl lysine -BSA

Specificity

This antibody reacts to lysine-acetylated proteins. No cross reactivity with nonacetylated lysine, and lysine with other modification.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Acetyl-Lysine Antibody (RM101) - Azide and BSA Free

Acetyl-Lysine Antibody (RM101) - Azide and BSA Free

Immunoprecipitation: Acetyl-Lysine Antibody (RM101) - Azide and BSA Free [NBP3-25979] -

Immunoprecipitation: Acetyl-Lysine Antibody (RM101) - Azide and BSA Free [NBP3-25979] - Immunoprecipitation of A431 cell lysates, using Clone RM101 at 1/500 dilution, was blotted with anti-PTEN mouse monoclonal antibody.
Acetyl-Lysine Antibody (RM101) - Azide and BSA Free

Western Blot: Acetyl-Lysine Antibody (RM101) - Azide and BSA Free [NBP3-25979] -

Western Blot: Acetyl-Lysine Antibody (RM101) - Azide and BSA Free [NBP3-25979] - Western blot of HeLa cells nontreated or treated with Trichostatin A (TSA), using Clone RM101 at 1/2000 dilution.
Acetyl-Lysine Antibody (RM101) - Azide and BSA Free

Multiplex Immunoassay: Acetyl-Lysine Antibody (RM101) - Azide and BSA Free [NBP3-25979] -

Multiplex Immunoassay: Acetyl-Lysine Antibody (RM101) - Azide and BSA Free [NBP3-25979] - Clone RM101 recognizes acetylated lysine in peptides with different sequences. It does not react with unmodified lysine.
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Applications for Acetyl-Lysine Antibody (RM101) - Azide and BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:100-1:500

ELISA

1:100 - 1:2000

Flow Cytometry

1:10 - 1:1000

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Immunohistochemistry

1:100 - 1:500

Immunoprecipitation

1:100-1:500

Western Blot

1:500 -1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Acetyl-Lysine

In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation. The reversible lysine acetylation of histones and non-histone proteins plays a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 deacetylases have been identified so far, but the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. Over 40 transcription factors and 30 other nuclear, cytoplasmic, bacterial, and viral proteins have been shown to be acetylated in vivo.

Alternate Names

acetyl Lysine, AC-LYS-OH, Lysine, N-acetyl lysine

Additional Acetyl-Lysine Products

Product Documents for Acetyl-Lysine Antibody (RM101) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Acetyl-Lysine Antibody (RM101) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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