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Acetyl-Lysine Antibody (RM101), Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NBP2-77397

Recombinant Monoclonal Antibody.
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NBP2-77397

Key Product Details

Species Reactivity

Validated:

All Species

Applications

Chromatin Immunoprecipitation (ChIP), ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM101

Concentration

1 mg/ml

Product Summary for Acetyl-Lysine Antibody (RM101)

Immunogen

Acetyl lysine -BSA

Specificity

This antibody reacts to lysine-acetylated proteins. No cross reactivity with nonacetylated lysine, and lysine with other modification.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Acetyl-Lysine Antibody (RM101)

Western Blot: Acetyl-Lysine Antibody (RM101) [NBP2-77397] - Analysis of HeLa cells nontreated or treated with Trichostatin A (TSA), using NBP2-77397.
Immunocytochemistry/Immunofluorescence: Acetyl-Lysine Antibody (RM101) [NBP2-77397] - Immunocytochemical staining of HeLa cells using anti-Acetyl-Lysine antibody (NBP2-77397, red). Actin filaments was labeled with fluorescein phalloidin (green), and nucleus stained with DAPI (blue).
Immunoprecipitation: Acetyl-Lysine Antibody (RM101) [NBP2-77397] - A431 cell lysates, using NBP2-77397, was blotted with anti-PTEN mouse monoclonal antibody.

Applications for Acetyl-Lysine Antibody (RM101)

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:100-1:500

ELISA

1:100 - 1:2000

Flow Cytometry

1:10 - 1:1000

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Immunohistochemistry

1:100 - 1:500

Immunoprecipitation

1:100-1:500

Western Blot

1:500 -1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50% Glycerol/PBS, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Acetyl-Lysine

In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation. The reversible lysine acetylation of histones and non-histone proteins plays a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 deacetylases have been identified so far, but the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. Over 40 transcription factors and 30 other nuclear, cytoplasmic, bacterial, and viral proteins have been shown to be acetylated in vivo.

Alternate Names

acetyl Lysine, AC-LYS-OH, Lysine, N-acetyl lysine

Additional Acetyl-Lysine Products

Product Documents for Acetyl-Lysine Antibody (RM101)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Acetyl-Lysine Antibody (RM101)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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