This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.

For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.

Applications for Acetyl-Lysine Antibody (7F8) [DyLight 755]

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot (Negative)

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Recommended applications are based on validated applications from the unconjugated base product NB110-96876. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Acetyl-Lysine

In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation. The reversible lysine acetylation of histones and non-histone proteins plays a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 deacetylases have been identified so far, but the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. Over 40 transcription factors and 30 other nuclear, cytoplasmic, bacterial, and viral proteins have been shown to be acetylated in vivo.

Alternate Names

acetyl Lysine, AC-LYS-OH, Lysine, N-acetyl lysine

Additional Acetyl-Lysine Products

Product Documents for Acetyl-Lysine Antibody (7F8) [DyLight 755]

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Acetyl-Lysine Antibody (7F8) [DyLight 755]

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

Acetyl-Lysine Antibody (7F8) [DyLight 755]

Novus Biologicals, part of Bio-Techne | Catalog # NB110-96876IR

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

Concentration

View all Acetyl-Lysine Antibodies »

Product Specifications

Immunogen

Localization

Specificity

Clonality

Host

Isotype

Description

Applications for Acetyl-Lysine Antibody (7F8) [DyLight 755]

ELISA

Immunohistochemistry

Western Blot (Negative)

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: Acetyl-Lysine

Alternate Names

Additional Acetyl-Lysine Products

Product Documents for Acetyl-Lysine Antibody (7F8) [DyLight 755]

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Acetyl-Lysine Antibody (7F8) [DyLight 755]

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