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Key Product Details

Species Reactivity

Validated:

Non-species specific

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7F8

Concentration

1 mg/ml

Product Summary for Acetyl-Lysine Antibody (7F8)

Immunogen

Acetylated KLH

Localization

Nucleus

Specificity

Detects proteins containing acetylated lysine residues in ELISA and western blots. Does not detect non-acetylated lysine residues.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Acetyl-Lysine Antibody (7F8)

Western Blot: Acetyl-Lysine Antibody (7F8) [NB110-96876] - Analysis of Acetyl-Lysine showing detection of Acetyl-Lysine protein using Mouse Anti-Acetyl-Lysine Monoclonal Antibody, Clone 7F8 . Primary Antibody: Mouse Anti-Acetyl-Lysine Monoclonal Antibody at 1:1000. (1) acetylated BSA (75ng of protein), (2) non-acetylated BSA, and (3) marker.
Immunohistochemistry: Acetyl-Lysine Antibody (7F8) [NB110-96876] - Immunohistochemistry analysis using Mouse Anti-Acetyl-LysineMonoclonal Antibody, Clone 7F8 (NB110-96876). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Acetyl-LysineMonoclonal Antibody (NB110-96876) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Backskin obtained from transgenic mice.

Applications for Acetyl-Lysine Antibody (7F8)

Application
Recommended Usage

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:1000
Application Notes
1 ug of Acetyl-Lysine Antibody was sufficient to detect acetylated chicken erythrocyte histones (sodium butyrate-treated) using 20 ug total protein, on western blot by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Acetyl-Lysine

Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Ne development (5).

Alternate Names

acetyl Lysine, AC-LYS-OH, Lysine, N-acetyl lysine

Additional Acetyl-Lysine Products

Product Documents for Acetyl-Lysine Antibody (7F8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Acetyl-Lysine Antibody (7F8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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