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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # D8H8

Concentration

1.0 mg/ml

Product Summary for A2BP1 Antibody (D8H8)

Immunogen

Mouse recombinant A2BP1 [Swiss-Prot# Q9JJ43]

Localization

Nucleus. Cytoplasm. Note: Predominantly nuclear.

Specificity

Does not cross react with paralogue FOX2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for A2BP1 Antibody (D8H8)

Western Blot: A2BP1 Antibody (D8H8) [NBP2-13169] - Western blot analysis of A2BP1 expression in 1) human brain and 2) mouse brain tissue lysates.
Immunocytochemistry/Immunofluorescence: A2BP1 Antibody (D8H8) [NBP2-13169] - A2BP1 antibody was tested in Neuro-2a cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry: A2BP1 Antibody (D8H8) [NBP2-13169] - IHC staining of A2BP1 in mouse brain using DAB with hematoxylin counterstain.

Applications for A2BP1 Antibody (D8H8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry

1:200

Immunohistochemistry-Frozen

reported in scientific literature (PMID 30001398)

Immunohistochemistry-Paraffin

1:200

Immunoprecipitation

1:10-1:100

Simple Western

1:20

Western Blot

1:1000
Application Notes
In Western Blot, a band is seen 50-55 kDa representing A2BP1. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 4 publications using NBP2-13169 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Tris-Glycine (pH 7.5) and 0.15M NaCl

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: A2BP1

A2BP1, also known as Ataxin-2-binding protein 1, Fox-1 homolog A and Rbfox1, is a member of the Fox-1 family, which is made up of RNA-binding proteins. A2BP1 regulates tissue-specific alternative splicing during erythropoiesis by locating the UGCAUG sequence and binding to the 5'-splice site while also preventing U2AF2 from binding to the 3' splicing site. A2BP1 may play a role in restricting the pathology of spinocerebellar ataxia type 2 (SCA2). The SCA2 gene produces ataxin-2 proteins which is a cause of familial neurodegenerative diseases. A2BP1 is primarily found in the nucleus but also seen in the cytoplasm and is expressed in the brain, heart, neurons, skeletal muscle, and embryo.

Alternate Names

2BP1, A2BP, A2BP1 Hexaribonucleotide-binding protein 1, Ataxin-2-binding protein 1, FOX-1, FOX1 hexaribonucleotide binding protein 1, Fox-1 homolog A, HRNBP1 ataxin 2-binding protein 1, RNA binding protein fox-1 homolog 1, RNA binding protein, fox-1 homolog (C. elegans) 1

Entrez Gene IDs

54715 (Human); 268859 (Mouse)

Gene Symbol

RBFOX1

Additional A2BP1 Products

Product Documents for A2BP1 Antibody (D8H8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for A2BP1 Antibody (D8H8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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