Skip to main content

8-oxo-dG Antibody, Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NB600-1508

Catalog #
Availability
Size / Price
Qty
Loading...
NB600-1508-50ul

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Canine

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Product Summary for 8-oxo-dG Antibody

Immunogen

8-Hydroxy-2'-deoxyguanosine and 8-hydroxyguanosine.

Reactivity Notes

Due to the conserved nature of the target cross reactivity would be expected in wide range of species. Mouse reactivity reported in scientific literature (PMID: 29559852).

Localization

Nuclear

Specificity

NB600-1508 cross reacts completely with 8-oxo-dG, and does not cross react with other naturally occurring nucleotides.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for 8-oxo-dG Antibody

ELISA: 8-OHdG Antibody [NB600-1508] - Competitive ELISA using the 8-OHG antibody demonstrates a high specificity towards 8-hydroxydeoxyguanosine (8-OHdG) and 8-hydroxyguanosine nucleotides. Partial cross-reactivity is observed with 8-mercaptoguanosine while 8-bromoguanosine shows some cross-reactivity. 2-Deoxyadenosine, 7-methylguanosine, guanosine monophosphate and guanosine show no reactivity.

Applications for 8-oxo-dG Antibody

Application
Recommended Usage

ELISA

1:100000-1:250000

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200
Application Notes
For IHC-Paraffin: It is suggested that the tissues are pretreated with 10 ug/mL of proteinase K for 40 minutes at 37 degrees C prior to staining. Dewaxed sections treated with 3%H2O2 in methanol for 30 minutes and blocking was with 10% serum in TBS. Sections were treated with proteinase K 10ug/ml in PBS pH 7.4, 40 minutes 37 degrees C prior to blocking. Detection is via DAB. Since the antibody is staining a nucleic acid it is important to use enough proteinase to penetrate the histone barrier.

Use in ICC/IF reported in scientific literature (PMID: 28269997).
Use in Western Blot reported in scientific literature (PMID:32269708).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 14 publications using NB600-1508 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Preservative

0.01% Thimerosal

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: 8-oxo-dG

The production of 8-Hydroxyguanine (8-oxo-dG) is almost exclusively elicited by oxidative stress. Polymerases preferentially insert adenine opposite 8-oxo-dG. Therefore, oxidatively damaged adducts, without repair, are susceptible to G to T transitions.

Long Name

8-Hydroxyguanine

Alternate Names

8-OHG, 8oxodG, 8-Hydroxyguanine

Additional 8-oxo-dG Products

Product Documents for 8-oxo-dG Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for 8-oxo-dG Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...
Loading...

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.