5T4 Antibody (39A11G5F2) - Chimeric - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-45123
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # 39A11G5F2
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
BALB/cAnNCrl mice or SJL/JorllcoCrl mice were immunized with either full length 5T4 or amino acid sequence 32-355 from 5T4 (Uniprot#: Q13641)
Specificity
This antibody not bind to the same epitope as 12B12C7C3.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Scientific Data Images for 5T4 Antibody (39A11G5F2) - Chimeric - BSA Free
Flow Cytometry: 5T4 Antibody (39A11G5F2) - Chimeric - BSA Free [NBP3-45123] -
Flow Cytometry: 5T4 Antibody (39A11G5F2) - Chimeric - BSA Free [NBP3-45123] - Paraformaldehyde-fixed MCF-7 cells permeabilized with 0.5% Triton were stained with the anti-unknown specificity antibody (isotype control, black line) or NBP3-45123 (blue line) at a dilution of 1:100 for 1 h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000, and the cells were analyzed using a FACSCanto flow-cytometer.
Immunocytochemistry/ Immunofluorescence: 5T4 Antibody (39A11G5F2) - Chimeric - BSA Free [NBP3-45123] -
Immunocytochemistry/ Immunofluorescence: 5T4 Antibody (39A11G5F2) - Chimeric - BSA Free [NBP3-45123] - Immunofluorescence analysis of paraformaldehyde-fixed A431 cells on coverslips stained with NBP3-45123 (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic, membrane, and vesicles staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-45123, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.Applications for 5T4 Antibody (39A11G5F2) - Chimeric - BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Functional
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: 5T4
Long Name
Oncofetal Antigen/Trophoblast Glycoprotein
Alternate Names
M6P1, TPBG
Gene Symbol
TPBG
Additional 5T4 Products
Product Documents for 5T4 Antibody (39A11G5F2) - Chimeric - BSA Free
Product Specific Notices for 5T4 Antibody (39A11G5F2) - Chimeric - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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