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5-Carboxylcytosine Antibody (RM462)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18829

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-18829

Key Product Details

Species Reactivity

Vertebrate

Applications

ELISA, Dot Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM462 expressed in HEK293

Concentration

1 mg/ml

Product Specifications

Immunogen

KLH-conjugated 5-Carboxylcytosine

Specificity

This antibody reacts to 5-Carboxylcytosine in DNA. No cross reactivity with non-modified cytosine and other modified cytosines in DNA.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit 5-Carboxylcytosine Antibody (RM462) (NBP3-18829) is a recombinant monoclonal antibody validated for use in ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for 5-Carboxylcytosine Antibody (RM462)

5-Carboxylcytosine Antibody (RM462)

Dot Blot: 5-Carboxylcytosine Antibody (RM462)[NBP3-18829]

Dot Blot: 5-Carboxylcytosine Antibody (RM462)[NBP3-18829]Dot blot of DNA using anti-5-caC Rabbit Monoclonal Antibody, Clone RM462.The membrane was pre-spotted with 50, 5, and 0.5 ng/dot of linear dsDNA (426 bp) containing either normal cytosines (unmodified), 5-carboxylcytosines (5-caC), 5-methylcytosines (5-mC), 5-hydroxymethylcytosines (5-hmC), or 5-formylcytosines (5-fC).
5-Carboxylcytosine Antibody (RM462)

ELISA: 5-Carboxylcytosine Antibody (RM462) [NBP3-18829] -

ELISA: 5-Carboxylcytosine Antibody (RM462) [NBP3-18829] - ELISA of DNA using Clone RM462 (1 ug/mL). The plate was coated with different concentrations of linear ssDNA (426 bp) containing either normal cytosines (unmodified), 5-methylcytosines (5-mC), 5-hydroxymethylcytosines (5-hmC), 5-carboxylcytosines (5-caC), or 5-formylcytosines (5-fC).

Applications for 5-Carboxylcytosine Antibody (RM462)

Application
Recommended Usage

Dot Blot

0.1 - 1 ug/ml

ELISA

0.2 - 2 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50% Glycerol/PBS, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: 5-Carboxylcytosine

5-carboxylcytosine (5caC) is the final oxidized derivative of 5-methylcytosine (5mC). 5mC is oxidized to 5-hydroxymethylcytosine (5hmC) which is then oxidized to 5-formylcytosine (5fC) then 5caC. Each of these oxidation steps are catalyzed by the Ten-Eleven Translocation (TET) family of enzymes. 5fC can then be further oxidized to 5-carboxylcytosine (5caC) by TET. Both 5fC and 5caC can be converted to unmodified cytosine by Terminal deoxynucleotidyl transferase (TdT) or Thymine DNA glycosylase (TDG) by base excision repair.

Alternate Names

5-caC

Additional 5-Carboxylcytosine Products

Product Documents for 5-Carboxylcytosine Antibody (RM462)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for 5-Carboxylcytosine Antibody (RM462)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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