5-Carboxylcytosine Antibody (RM462)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18829
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Vertebrate
Applications
ELISA, Dot Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM462 expressed in HEK293
Concentration
1 mg/ml
Product Specifications
Immunogen
KLH-conjugated 5-Carboxylcytosine
Specificity
This antibody reacts to 5-Carboxylcytosine in DNA. No cross reactivity with non-modified cytosine and other modified cytosines in DNA.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit 5-Carboxylcytosine Antibody (RM462) (NBP3-18829) is a recombinant monoclonal antibody validated for use in ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for 5-Carboxylcytosine Antibody (RM462)
Dot Blot: 5-Carboxylcytosine Antibody (RM462)[NBP3-18829]
Dot Blot: 5-Carboxylcytosine Antibody (RM462)[NBP3-18829]Dot blot of DNA using anti-5-caC Rabbit Monoclonal Antibody, Clone RM462.The membrane was pre-spotted with 50, 5, and 0.5 ng/dot of linear dsDNA (426 bp) containing either normal cytosines (unmodified), 5-carboxylcytosines (5-caC), 5-methylcytosines (5-mC), 5-hydroxymethylcytosines (5-hmC), or 5-formylcytosines (5-fC).ELISA: 5-Carboxylcytosine Antibody (RM462) [NBP3-18829] -
ELISA: 5-Carboxylcytosine Antibody (RM462) [NBP3-18829] - ELISA of DNA using Clone RM462 (1 ug/mL). The plate was coated with different concentrations of linear ssDNA (426 bp) containing either normal cytosines (unmodified), 5-methylcytosines (5-mC), 5-hydroxymethylcytosines (5-hmC), 5-carboxylcytosines (5-caC), or 5-formylcytosines (5-fC).Applications for 5-Carboxylcytosine Antibody (RM462)
Application
Recommended Usage
Dot Blot
0.1 - 1 ug/ml
ELISA
0.2 - 2 ug/ml
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50% Glycerol/PBS, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: 5-Carboxylcytosine
Alternate Names
5-caC
Additional 5-Carboxylcytosine Products
Product Documents for 5-Carboxylcytosine Antibody (RM462)
Product Specific Notices for 5-Carboxylcytosine Antibody (RM462)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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