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4-amino Biphenyl DNA Antibody (4C11), Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NB100-415

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NB100-415
NB100-415SS

Key Product Details

Species Reactivity

Validated:

Mouse, All Species

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse Clone # 4C11

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Product Summary for 4-amino Biphenyl DNA Antibody (4C11)

Immunogen

4-amino Biphenyl DNA

Reactivity Notes

Detects DNA from all species. Mouse reactivity reported in scientific literature (PMID: 12700401).

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Scientific Data Images for 4-amino Biphenyl DNA Antibody (4C11)

Immunohistochemistry: 4-amino Biphenyl DNA Antibody (4C11) [NB100-415] - Human breast tumor tissue with high (A) and low (B) adduct levels. Images courtesy of Dr. Regina Santella.

Applications for 4-amino Biphenyl DNA Antibody (4C11)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:50 1:100

Immunohistochemistry

1:50-1:100
Application Notes
This 4-amino Biphenyl DNA (4C11) antibody is useful for ELISA, Immunocytochemistry/Immunofluorescence and Immunohistochemistry.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 4 publications using NB100-415 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 4-amino Biphenyl DNA

4-amino Biphenyl (4-ABP) is an arylamine (aromatic amine) compound and as one of the major carcinogenic constituents of tobacco smoke other environmental sources, it poses critical health hazards to human beings. Under the action of carcinogen activating biotransformation enzymes such as cytochrome P4501A2, 4-ABP gets metabolized to generate a direct-acting mutagen known as N-hydroxy-aminobiphenyl. Nevertheless, the accumulation of biologically active metabolites is dependent on the balance between activation and detoxification enzymes. 4-ABP has been linked to bladder and breast cancers in human beings as well as in rodent models. Our 4-amino Biphenyl DNA antibody (4C11) is a monoclonal antiserum which recognizes 4-aminobiphenyl (4-ABP)-DNA adducts and it has been characterized by competitive enzyme-linked immunosorbent assay (ELISA), immunohistochemistry (IHC) and Immunocytochemistry/Immunofluorescence (ICC/IF) techniques. This antibody does not recognize the DNA adducts of several other aromatic amines tested including 1-aminopyrene, 8-nitro-1-aminopyrene, and 6-nitro-1-aminopyrene.

Alternate Names

4-AMBP DNA, 4-aminobiphenyl DNA, 4-amino-Biphenyl DNA

Additional 4-amino Biphenyl DNA Products

Product Documents for 4-amino Biphenyl DNA Antibody (4C11)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for 4-amino Biphenyl DNA Antibody (4C11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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