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Key Product Details

Species Reactivity

Validated:

Human, All Species

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # P3UI

Concentration

2.6 mg/ml

Product Summary for 3-OHKYN Antibody (P3UI)

Immunogen

3-OHKYN modified KLH

Localization

Cytoplasmic

Specificity

This is specific for 3-hydroxykynureinine (3OH-Kyn) modifications on proteins. It is likely to react with 3OH-Kyn modification on lysine, histidine and cysteine residues on proteins. It does not react with enzymes involved in the degradation of tryptopha

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for 3-OHKYN Antibody (P3UI)

Western Blot: 3-OHKYN Antibody (P3UI) [NB100-597] - Detection of 3'OHKYN in modified BSA using NB 100-597. 10 second ECL exposure. Lane 1: BSA (-) Lane 2: BSA (+).
Immunohistochemistry-Paraffin: 3-OHKYN Antibody (P3UI) [NB100-597] - 3-OHKYN was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human 3-OHKYN (P3UI) Monoclonal Antibody (Catalog # NB100-597) at 1:300 for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in neurons.

Applications for 3-OHKYN Antibody (P3UI)

Application
Recommended Usage

ELISA

reported in scientific literature

Immunocytochemistry/ Immunofluorescence

1:100-1:200

Immunohistochemistry-Paraffin

reported in scientific literature

Western Blot

2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 1 publication using NB100-597 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Tris-Glycine and 0.15M NaCl

Preservative

0.05% Sodium Azide

Concentration

2.6 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: 3-OHKYN

3-OHKYN (3-Hydroxykynurenine) is tryptophan's toxic metabolite which is capable of inducing oxidative damage as well as cell death consequently contributing to different neurological and psychiatric disorders. Tryptophan is either transaminated to kynurenate (an endogenous glutamate receptor antagonist), or hydroxylated to 3-OHKYN which is degraded to excitotoxic N-methyl-D-aspartate (NMDA) agonist, quinolinate. Under physiological conditions, kynurenine pathway is activated to form metabolic precursor NAD+; however, under inflammatory, oxidative or neurotoxic attack, there is an increased activation of indoleamine dioxygenase (IDO), kynurenine monooxygenase (KMO) and tryptophan dioxygenase (TDO), yielding the formation of toxic metabolites such as 3-OHKYN and quinolinic acid. Once 3-OHKYN crosses neuronal cell membranes through neutral amino acid transporter (NAT), it can follow two routes: (i) may act as ROS scavenger/antioxidant via direct scavenging or by triggering integral antioxidant responses evoked by transcription signaling pathways, such as the Nrf2/Keap1/ARE axis (ii) 3-OHKYN may undergoes autooxidation yielding highly reactive molecules, such as o-aminoquinone, which in turn causes exacerbated oxidative damage, activation of deadly cascades, and massive cell death.

Alternate Names

3039Hydroxykynurenine, 3-hydroxykynuenine modification

Additional 3-OHKYN Products

Product Documents for 3-OHKYN Antibody (P3UI)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for 3-OHKYN Antibody (P3UI)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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