Skip to main content

Key Product Details

Species Reactivity

Validated:

Cynomolgus Monkey

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for 14-3-3 beta Antibody

Immunogen

Produced in rabbits immunized with purified, recombinant Cynomolgus 14-3-3 beta (Uniprot#: Q4R572-2; Met2-Asn244).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for 14-3-3 beta Antibody

Western Blot: 14-3-3 beta Antibody [NBP2-98453] - Anti-14-3-3 beta rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lane C: NCI-H1299 Whole Cell Lysate. Lane D: A431 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 28 kDa. Observed band size: 32 kDa (We are unsure as to the identity of these extra bands).
Immunoprecipitation: 14-3-3 beta Antibody [NBP2-98453] - 14-3-3 beta was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. Lane B: 0.5 mg Hela Whole Cell Lysate. 4 ul anti-14-3-3 beta rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-14-3-3 beta rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 32 kDa. Observed band size: 32 kDa

Applications for 14-3-3 beta Antibody

Application
Recommended Usage

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 14-3-3 beta

14-3-3 proteins constitute a highly conserved family of homo- and heterodimeric proteins present in high abundance in all eukaryotic cells. 14-3-3 proteins act as key regulators of intracellular signal transduction through their ability to bind specific motifs containing phosphorylated serine or threonine residues. There are at least seven distinct 14-3-3 genes in vertebrates: alpha/beta, epsilon, eta, gamma, theta, sigma, and zeta/delta.

Alternate Names

1433 beta, GW128, HS1, YWHAB

Gene Symbol

YWHAB

Additional 14-3-3 beta Products

Product Documents for 14-3-3 beta Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for 14-3-3 beta Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...