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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1C3

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Summary for 12-Lipoxygenase Antibody (OTI1C3) - Azide and BSA Free

Immunogen

Full length human recombinant protein of human ALOX12 (NP_000688) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

75.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for 12-Lipoxygenase Antibody (OTI1C3) - Azide and BSA Free

Western Blot: 12-Lipoxygenase Antibody (OTI1C3) - Azide and BSA Free [NBP2-70165] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY 12-Lipoxygenase.
Immunohistochemistry: 12-Lipoxygenase Antibody (OTI1C3) - Azide and BSA Free [NBP2-70165] - Analysis of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: 12-Lipoxygenase Antibody (OTI1C3) - Azide and BSA Free [NBP2-70165] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Applications for 12-Lipoxygenase Antibody (OTI1C3) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: 12-Lipoxygenase

Oxygenase and 14,15-leukotriene A4 synthase activity

Long Name

Polyunsaturated fatty acid lipoxygenase ALOX12

Alternate Names

12LO, 12S-lipoxygenase, 12S-LOX, ALOX12, EC 1.13.11.-, EC 1.13.11.31, EC 1.13.11.33, EC 3.3.2.-, LOG12

Gene Symbol

ALOX12

Additional 12-Lipoxygenase Products

Product Documents for 12-Lipoxygenase Antibody (OTI1C3) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for 12-Lipoxygenase Antibody (OTI1C3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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