Universal Sulfotransferase Activity Kit

The Universal Sulfotransferase Activity Kit (Catalog # EA003) provides a simple, non-radioactive high-throughput compatible format for assaying the enzyme activity of all sulfotransferases that use 3-phosphoadenosine-5-phosphosulfate (PAPS) as the donor substrate. This kit utilizes inositol monophosphatase 3 (IMPAD1) as a coupling phosphatase to remove inorganic phosphate quantitatively from the leaving nucleotide 3-phosphoadenosine-5-phosphate (PAP) that is generated during sulfotransferase reactions.

Features

• Applicable for all sulfotransferases that use PAPS as the donor substrate
• Non-radioactive
• No time-consuming separation steps (e.g. column chromatography)
• Provides accurate kinetic analysis of sulfotransferase reactions
• Amenable to high-throughput analysis

Kit Contents

• CHO cell-expressed Recombinant Mouse IMPAD1
• PAP
• Phosphatase Buffer 3
• Phosphate Standard
• Malachite Green Reagents A & B

Data Example

Activity of CHST7

The ability of Recombinant Mouse Carbohydrate Sulfotransferase 7/CHST7 (Catalog # 5108-ST) to transfer sulfate from PAPS to N-acetyl glucosamine was measured using the Universal Sulfotransferase Activity Kit (Catalog # EA003). The corrected optical densities were plotted versus the amount of enzyme. Specific activity of CHST7 was calculated to be 116.4 pmol/min/μg.