Peroxidase Activity Assay Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24528

Novus Biologicals, part of Bio-Techne
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NBP3-24528
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Key Product Details

Sample Type & Volume Required Per Well

Plant tissue

Sensitivity

0.01 U/mL

Assay Range

0.01 - 100 U/mL

View all Peroxidase Activity Assays »

Product Specifications

Kit Type

Activity Assay Kit (Colorimetric)

Reactivity

Non-species specific

Description

Detection instrument: Microplate reader (410 nm - 430 nm, optimum wavelength: 420 nm)
Assay time: 60 min

Precision

Intra-Assay Precision (Precision within an assay) %CV < 3.2

Inter-Assay Precision (Precision between assays) %CV < 5

Recovery for Peroxidase Activity Assay Kit (Colorimetric)

Recovery

Average recovery rate: 102%

Scientific Data Images for Peroxidase Activity Assay Kit (Colorimetric)

Peroxidase Activity Assay Kit (Colorimetric)

Peroxidase Activity Assay Kit (Colorimetric) [NBP3-24528] -

Peroxidase Activity Assay Kit (Colorimetric) [NBP3-24528] - Standard Reference Curve
Peroxidase Activity Assay Kit (Colorimetric)

Peroxidase Activity Assay Kit (Colorimetric) [NBP3-24528] -

Peroxidase Activity Assay Kit (Colorimetric) [NBP3-24528] - Assay Example

Kit Contents for Peroxidase Activity Assay Kit (Colorimetric)

  • Microplate
  • Plate Sealer
  • Reagent 1: Buffer Solution
  • Reagent 2: Chromogenic Agent
  • Reagent 3: Substrate Solution
  • Reagent 4: Stop Solution

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Peroxidase

Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue. HRP binds 1 heme B (iron-protoporphyrin IX) group and 2 calcium ions per subunit. Subunits are monomeric.

Alternate Names

EC 1.11.1.7, Horseradish Peroxidase, HPRC 1, HPRC1, HRPA2, HRPC 2, HRPC2, HRPE5, HRPN, Neutral peroxidase, Peroxidase A2, Peroxidase C1A, Peroxidase C1B, Peroxidase C2, Peroxidase C3, Peroxidase E5, Peroxidase N, PRXC1A, PRXC1B, PRXC2, PRXC3

Additional Peroxidase Products

Product Documents for Peroxidase Activity Assay Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Peroxidase Activity Assay Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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