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Dopamine ELISA Kit (Colorimetric)

Catalog # KA3838 | Novus Biologicals a Bio-Techne Brand
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KA3838

Key Product Details

Sample Type & Volume Required Per Well

Biological Fluid (1-750 uL)

Sensitivity

0.25 pg/mL x Correction factor

Assay Range

0.5 ng/ml to 80 ng/ml

Product Specifications

Assay Type

Colorimetric

Kit Type

ELISA Kit (Colorimetric)

Conjugate

HRP

Reactivity

Human, Rat, Fish, Insect

Specificity

Dopamine ELISA Kit is an ultra-sensitive enzyme immunoassay for the quantitative determination of Dopamine in biological fluids.

Description

Dopamine ELISA Kit is an ultra-sensitive enzyme immunoassay for the quantitative determination of Dopamine in biological fluids.

Precision

Intra-Assay Precision (Precision within an assay) Human EDTA-Plasma: 15.6%, Cell Culture Medium: 18.4%

Recovery for Dopamine ELISA Kit (Colorimetric)

Recovery

Human EDTA-Plasma: 97.7%, Cell Culture Medium: 98.6%

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4°C.

Background: Dopamine

Dopamine is extracted by using a cis-diol-specific affinity gel, acylated and then derivatized enzymatically. The competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analyte compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations.

Alternate Names

3 4 dihydroxyphenethylamine, 3 hydroxytyramine, DA, Dopamine, Oxytyramine

Product Documents for Dopamine ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Dopamine ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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