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Antibodies for Western Blot

Primary Antibodies

Bio-Techne® offers a large portfolio of primary antibodies validated for Western blot experiments.

Secondary Antibodies

Bio-Techne offers enzyme- and fluorochrome-conjugated secondary antibodies for use in Western blot experiments.

Epitope Tag Antibodies

Bio-Techne offers antibodies for the most commonly used epitope tags for the detection of tagged proteins with Western blot.

Novus Biologicals Brand Logo

Novus Biologicals Western Blot Tools

Novus Biologicals offers reagents for your entire Western blot workflow including over 65,000 Western blot-validated antibodies, HRP-conjugated secondary antibodies, detection reagents, and controls such as blocking peptides, control lysates, and over-expression lysates.

Western Blot Imaging and Analysis

FluorChem R – Infrared Fluorescence Western Blot Imager

FluorChem™ Imagers

Bio-Techne’s FluorChem imagers are all-in-one solutions for high-quality multimodal imaging of Western blot membranes and electrophoresis gels.

AlphaImager HP

AlphaImager® Systems

Bio-Techne’s AlphaImager systems are digital gel imagers and documentation systems for colorimetric and fluorescent research applications.

AlphaView Software

AlphaView® Software

Bio-Techne’s AlphaView software allows for the easy capture, analysis, and annotation of membrane and gel images. Optimized for use with our FluorChem and AlphaImager systems.

Simple Western™ Systems

Jess Simple Western instrument by ProteinSimple, a Bio-Techne brand

Jess

Bio-Techne’s Jess is an automated capillary-based Western blotting system. Its capabilities include chemiluminescent immunodetection, two-color fluorescence, and protein normalization.

Wes Simple Western instrument by ProteinSimple, a Bio-Techne brand

Wes

The predecessor to Jess, Bio-Techne’s automated, capillary-based Western blotting system, Wes, separates and analyzes proteins by chemiluminescence.

Sally Sue Simple Western instrument ProteinSimple, a Bio-Techne brand

Sally Sue

Bio-Techne's Sally Sue lets you separate and analyze proteins by size in an automated fashion. Only 5 µL of sample is needed, and up to 96 samples can be run in one experiment.

Peggy Sue Simple Western instrument ProteinSimple, a Bio-Techne brand

Peggy Sue

Bio-Techne’s Peggy Sue system lets you separate and analyze proteins by either size or charge in an automated fashion. Only 5 µL of sample is needed, and up to 96 samples can be run in one experiment.

Nanopro 1000 Simple Western instrument ProteinSimple, a Bio-Techne brand

NanoPro 1000

The NanoPro 1000 is Bio-Techne’s automated, capillary-based nano-immunoassay platform that uses isoelectric focusing to characterize protein post-translational modifications.

Compass Software

Compass Software

Analyze Simple Western size or charge assay data with Bio-Techne’s Compass software. Equipped with features that satisfy the 21 CFR Part 11 data security requirements.

Single-Cell Westerns on the Milo Instrument

Perform Western Blotting in Single Cells

Our single-cell Western platform measures protein expression in ~1,000 single cells in a single run.

Related Western Blot Resources

Western Blot Handbook

This step-by-step Western blot guide from Novus Biologicals is a great introduction to Western blot. It provides detailed information for understanding, performing, and troubleshooting a Western blot protocol.

Western Blot Troubleshooting Resource

A comprehensive, online resource for troubleshooting the most common problems of Western blot including no signal, multiple bands, and high background.

Development of Validated Monoclonal Antibodies

This white paper describes our validation process, including antigen design, feasibility testing, specificity and sensitivity testing, and quality control and release decisions, for our monoclonal antibodies.

Background Information