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Ponceau S Staining Solution

Catalog # 5225 | Tocris Bioscience a Bio-Techne Brand
Stain used to detect protein bands after Western blotting
Stain used to detect protein bands after Western blotting
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Key Product Details

Description: Stain used to detect protein bands after Western blotting
Chemical Name: 3-Hydroxy-4-[2-[2-sulfo-4-[2-(4-sulfophenyl)diazenyl]phenyl]diazenyl]-2,7-naphthalenedisulfonic acid tetrasodium salt
Molecular Weight: 760.57

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Customer Reviews for Ponceau S Staining Solution (3)

4.3 out of 5
3 customer ratings
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  • Stain to detect protein bands and confirm sucessful transfer prior to antibody incubation.
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: HEK293
    Protein samples were resolved in NuPAGE 4–12% Bis-Tris gel (Invitrogen) and transferred to 0.2 µm PVDF membrane. The membrane was stained with Ponceau S solution for less than a minute to detect protein bands and confirm successful sample run and transfer (lane 2-6) or not (lane 1).
    The stain on the membrane can be washed off with PBS or TBS so that it can be further incubated with the primary antibody for immunodetection.
    Ponceau S Staining Solution Serum 5225
  • Best staining for tracking protein transfer in western blot
    Name: Luiz Alexandre Magno
    Species: Mouse
    Assay Type: In Vitro
    We use Ponceau S (Cat. No. 5225) to confirm successful transfer of proteins from the gel onto nitrocellulose membranes during the transfer step of western blot analysis. This reagent stains protein bands very quickly (less than 1 min) and has the big advantage to be easily destained after a quick wash step that can be made with water or PBS. It is also used in our lab to detect protein degradation or any other abnormalities like overloading (as shown in the figure, the line #9 contains a protein sample degrade). Moreover, it is also useful to select the membrane area to be cut after transfer, based on the migration pattern of the protein standards (line #1). This enables one to incubate the different membrane sections with various primary antibodies.
    1. To get a clear background, wash the membrane quickly in PBS after staining2. Ponceau red stain is not stable and will disappear if you destain longer.3. It is advisable to mark with a pencil the position of the bands of interest if you want to cut a membrane in various sections
    Ponceau S Staining Solution Serum 5225
  • Western blot confirmation
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Western blot confirmation

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Product Documents for Ponceau S Staining Solution

Certificate of Analysis

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