Simple Plex Assays for Viral Titer

Understanding the number of particles, or infectious units, in your viral prep is essential, as it directly determines the viral transduction efficiency (i.e., how effectively the viruses will deliver genetic material into the target cells) and the resulting number of integrated gene copies. Accurate viral titration is a cornerstone of viral transduction workflows, ensuring that sufficient viral preps have been performed to achieve the desired transduction efficiency and generate reliable results during cell transfection experiments.

Simple Plex Viral Titer assays, available on the Ella platform, provide a fast, reliable, and reproducible solution for viral titration. These assays streamline the process, minimizing variability while delivering precise quantification of viral capsid proteins across a wide dynamic range. Whether you are working with adeno-associated viruses (AAV) or lentivirus, Simple Plex offers assays tailored for both, making it easier to optimize workflows and ensure the success of your gene delivery or cell therapy applications.

Simple Plex AAV Assays are paired with PROGEN AAV Antibodies 

For application of AAV vectors in gene therapy, the determination of total capsid titer is essential. PROGEN AAV Titration ELISAs are well-known for their ability to provide reliable and accurate quantification of AAV total capsid titers, based on the proven sensitivity and specificity of PROGEN AAV antibodies for AAV intact particles. The Simple Plex AAV assays offer the same proven specificity as the industry-leading PROGEN ELISA along with the convenience, sample-conserving, and timesaving advantages inherent in the fully automated Ella platform.

All steps of the Simple Plex AAV assays on Ella are highly automated thanks to the microfluidic cartridge—everything is preloaded, even the calibration curve. Just pipette your diluted samples onto the cartridge, add wash buffer, press start in the Ella Runner Software. In addition, each Simple Plex AAV cartridge includes a fully built-in and pre-validated standard curve. This eliminates the need to prepare, plate, or serially dilute standards—reducing hands-on time, removing a major source of operator variability, and helping ensure consistent, reproducible quantitation across assays, cartridges, and users.

Inside the microfluidic cartridge are glass nano reactors (GNRs), the core of the Simple Plex Assay. In Simple Plex AAV Assays, the GNRs are coated with the PROGEN antibodies that work to capture intact AAV capsids. That same corresponding AAV antibody is also lyophilized in the cartridge to act as the detection reagent. The Ella Platform’s fluorescence detection system enables detection across a broad dynamic range, minimizing the need to run multiple dilutions to find the ideal MRD. The efficient cartridge design, featuring three independent GNRs per sample inlet, eliminates the need to run samples in duplicate. In total, from set up to fully processed and analyzed results takes less than 90 minutes.

Simple Plex AAV assays use the same high-quality antibodies as PROGEN ELISAs. The Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) are indicated for each assay in capsids per milliliter.

Ella satisfies the demands of timely data generation for maintaining efficient production and overcomes the lengthy investigations and sample retests that often result from human error. You also get excellent assay sensitivity and highly reproducible results, which helps ensure you get the high-quality data required to meet regulatory standards for titration throughout the viral vector production workflow. 

Related Products