Simple Plex AAV1 Cartridge

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-OT-007969

Simple Plex AAV1 assay kit contains cartridge, sample diluent SD19, and wash buffer.
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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Bioprocess Samples (25 µL)

Sensitivity

0.59 106 capsids/mL

Assay Range

2.62-100000 106 capsids/mL (Bioprocess Samples)

Simple Plex AAV1 Cartridge Summary

Simple Plex assay for the detection of Adeno-Associated Virus type 1 (AAV1) intact capsids in bioprocess samples. This assay uses PROGEN's AAV1 (ADK1a) antibody.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all AAV1 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Reactivity

Viral

Specificity

This assay recognizes intact AAV1 capsids. The AAV1 antibody (ADK1a) used in this assay cross-reacts with AAV6 and AAV12.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

Bioprocess Samples

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 31 33
Mean (10<sup>6</sup> capsids/mL) 27.5 1291 28.0 1242
Standard Deviation 0.771 52.8 2.38 79.2
CV% 2.8 4.1 8.5 6.4

Linearity

Samples containing and/or spiked with high concentrations of AAV1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Images for Simple Plex AAV1 Cartridge

AAV1 Simple Plex Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: AAV1

Adeno-Associated Virus Subtype 1 (AAV1) is a vector often used in gene delivery systems.

Long Name

Adeno-associated Virus 1

Additional AAV1 Products

Product Documents for Simple Plex AAV1 Cartridge

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex AAV1 Cartridge

For research use only

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