Microglial activation drives neuroinflammatory signaling, yet most assays capture only a narrow slice of the cytokine network. This application note showcases a multi-platform workflow combining R&D Systems™ Luminex Multiplex Assays, Simple Plex™ Assays on the Ella™ Platform, and Simple Western™ Analysis on Jess™ Platform to resolve how IL-10 reshapes microglial responses to IFN-γ/LPS stimulation.
Cytokine and Chemokine Profiling in Microglia
Using BV-2 and primary mouse microglia, the study profiles 45 cytokines and chemokines, revealing IL-10–mediated suppression of pro-inflammatory signals and induction of IL-1ra.
Mechanistic Insight Through Cross-Platform Validation
Cross-platform validation and STAT1/STAT3 phosphorylation analysis provide mechanistic insight, enabling a streamlined approach for studying neuroinflammatory pathways and evaluating modulators of microglial function.
