- Research Areas
- Clinical Solutions
- Contact Us
Clot formation is triggered and inhibited by a cascade of coagulation factors, proteases, and regulators. The extrinsic pathway is initiated by contact between blood borne factors and subendothelial Tissue Factor. The intrinsic pathway is triggered by contact of these factors with negatively charged surfaces. Both pathways result in the activation of Coagulation Factor X.
The backbone of the coagulation cascade is a system of serine proteases (coagulation factors and plasminogen activators) which are held in check by protease inhibitors (Serpins and Protein S). The endpoint of cascade activation is the cleavage of Fibrinogen by Thrombin and the crosslinking of Fibrin into the clot.
The activation of platelets at sites of endothelial damage results in the release of many coagulation cascade factors and regulators. In addition, activated platelets express adhesion proteins which promote platelet aggregation and clot formation.
These assay platforms let you detect many proteins in one experiment. They cover secreted factors, shed soluble receptors, and intracellular molecules. These assays are optimized for high sensitivity, low background, and minimal cross-reactivity.
Meet our protein characterization problem solvers – Simple Western™, Maurice, Simple Plex™, Single-Cell Western, MFI™ Systems, and FluorChem™ Systems.