Properties and Applications of MitoBrilliant Dyes
MitoBrilliant probes have been developed to overcome some common limitations encountered with standard mitochondrial trackers. The range includes 2 types of dyes, MitoBrilliant Live dyes, suitable for live-cell imaging; and a ‘universal’ dye, suitable for both live-cell and fixed-cell imaging.
Excellent Brightness and Photostability
Δψm-Dependent and Δψm-Independent Mitochondrial Staining
- MitoBrilliant Live dyes (Cat. No. 7417 and 7693) accumulate in the mitochondria of live cells in a Δψm dependent manner.
- MitoBrilliant 646 (Cat. No. 7700) can be retained in mitochondria when Δψm is disrupted.
Improved Mitochondrial Staining Fidelity Post-Fixation
MitoBrilliant 646 (Cat. No. 7700) staining is retained after fixation with PFA and provides significantly improved staining resolution than a leading competitor dye.
MitoBrilliant Dyes Support a Wide Range of Applications
MitoBrilliant dyes enable highly accurate, sensitive, specific and persistent labeling of mitochondria in a wide variety of applications.
MitoBrilliant Technology - Key Features
| Product Name | Abs/Em (nm) | Δψm Dependent | Live/Fixed Cell Use? | Image Without Wash Step? | Demonstrated Applications |
|---|---|---|---|---|---|
| 655/668 | No | Suitable for live and fixed cell work | Yes, but replacing media recommended | Fixed-cell imaging, Live-cell imaging Flow cytometry, IHC/ICC Super-resolution microscopy (STED), High-content screening | |
| 648/662 | Yes | Live-cell work only | Yes, but replacing media recommended | Live-cell imaging, Flow cytometry, High-content screening | |
| 550/568 | Yes | Live-cell work only | Yes, but replacing media recommended | Live-cell imaging, Flow cytometry, High-content screening |
Customer Success Stories
Researcher at CMMIT, Murdoch University, Australia
MitoBrilliant Product Guide
Our guide provides protocols and data for use in different research applications including flow cytometry, immunohistochemistry, live-cell and fixed-cell imaging, and super-resolution microscopy. Please download for more information.