Supremely Flexible. Highly Reliable.
DuoSet™ ELISA Development Systems kits offer a reliable, economical alternative to purchasing a ready-to-use ELISA or separate antibodies. Each DuoSet kit contains carefully selected and validated matched antibody pairs for accurate measurement of recombinant and natural proteins in biological fluids.
A mass-calibrated recombinant standard helps reduce assay variability and ensure long-term consistency.
- Highly specific and optimized for superior performance
- Adaptable across multiple platforms
- More than 1,000 targets with new and novel targets across multiple species
- Low cost per data point
Proven Quality. Consistent Performance.
Specific and Consistent
Carefully selected antibody pairs combined with a mass-calibrated standard help ensure accurate performance and consistent results for data collected over time.
Large Menu Across Species
Select from an expansive DuoSet kit menu that includes more than 1,000 ELISAs across 16 different species.
DuoSet ELISA Development Systems Assay Principle
DuoSet IC (Intracelluar) ELISA Development Systems
DuoSet IC (Intracellular) kits offer a fast, sensitive and economical method to detect and quantify intracellular factors in cell lysate samples. These signal transduction assays offer an attractive alternative to traditional Western Blot, providing up to 10 to 30 times more sensitivity.
DuoSet IC (Intracellular) Phospho-specific ELISA kits measure the amount of phosphorylated protein in cell lysates using activity using a specifically targeted detection antibody included in the kit. Targets include kinases, heat shock, apoptosis-related and genotoxic stress proteins.
DuoSet Ancillary Reagent Kits
Supplemental products are available in a convenient Ancillary Reagent Kit for performing five 96-well assays. Each DuoSet Ancillary Reagent Kit provides a comprehensive collection of reagents and plates to assay five 96-well DuoSet ELISA plates. Choose components separately (in bulk sizes) for easy assay customization.
DuoSet Ancillary Reagent Kits include:
- 96-well Plates
- Plate Sealers
- Color Reagent A
- Color Reagent B
- Stop Solution
- ELISA Plate-coating Buffer
- Reagent diluent
- Wash Buffer
Customer Success Stories
First Flight Biosciences
"Using this ELISA kit to test the ErbB2 level of CHO cell at different conditions. Very sensitive and easy to use."
University of Hohenheim
"Performance and reproducibility of the kit are very good. Also, very good customer service and fast shipment."
Kansas State University
"I use this for several different ELISAs. The DuoSet has worked well for every ELISA I have run."
"Very clear instruction and everything is provided with the kit. Very satisfied."
University of California, San Diego
"Great DuoSet kit as always. Fantastic value. Tested in both serum and urine; produced great results."
DuoSet ELISA Kit Applications
Explore novel targets with customizable DuoSet ELISA kits. Uncover new views into the role of proteins in cancer and accelerate protein-based therapeutics.
Animal Model Systems
Animal Model Systems
Choose from a large selection of DuoSet ELISA kits with analytes supporting a range of biological processes and 16 different model species.
Learn How to Run a DuoSet ELISA Assay
Get step-by-step guidance on how to run DuoSet ELISA kit. DuoSet ELISA Development Systems include the basic components required to develop a sandwich ELISA, including matched antibody pairs, standard, and streptavidin HRP.
Your Comprehensive Guide to ELISAs
Explore Bio-Techne's wide range of ELISAs and gain deeper insight into types of ELISAs, ELISA controls, best practices, and troubleshooting. Learn about detection of molecules, sample preparation, and calculating results.