Do you have data for concentration for mouse injection (in-vivo)?

Name: VPR-66, ROR gamma T and ROR alpha Inhibitor (ROR gamma T and ROR alpha Inhibitor)
Brand: Novus Biologicals
SKU: NBP2-29335

We don't have any data on in vivo use of the inhibitor, nor know of any publications. However, we would recommend a starting dose of 20 mg/kg and going up in dosage from there. You would need to empirically determine the suitability of the inhibitor for your mouse model system as well as optimize the dosage. The compound can be stored long term at -80.

VPR-66, ROR gamma T and ROR alpha Inhibitor (ROR gamma T and ROR alpha Inhibitor)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29335

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Product Specifications

Application Notes

The ROR gamma T inhibitor is readily soluble in DMSO. This inhibitor should be dissolved in DMSO, ethanol, or other similar organic solvents. The solubility in DMSO exceeds 10 mg/ml. The aqueous solubility is greater than 0.5 mg/ml. Previous studies have used 10 mM stock solutions in DMSO. Prepare concentrated intermediate dilutions in DMSO and then add small volumes to buffer or cell culture media to provide the final aqueous working concentrations. It is not recommended to prepare serial dilutions in aqueous media.

Scientific Data Images

VPR-66, ROR gamma T and ROR alpha Inhibitor (ROR gamma T and ROR alpha Inhibitor) [NBP2-29335] - VPR-66, ROR gamma T and ROR alpha Inhibitor (ROR gamma T and ROR alpha Inhibitor) Chemical structure of the ROR gamma T inhibitor VPR-66: C16H21N3O2

VPR-66, ROR gamma T and ROR alpha Inhibitor (ROR gamma T and ROR alpha Inhibitor) [NBP2-29335] - VPR-66, ROR gamma T and ROR alpha Inhibitor (ROR gamma T and ROR alpha Inhibitor)VPR-66 inhibition of transcriptional activity determined in CHO cells transfected with ROR gamma.

VPR-66, ROR gamma T and ROR alpha Inhibitor (ROR gamma T and ROR alpha Inhibitor) [NBP2-29335] - VPR-66, ROR gamma T and ROR alpha Inhibitor (ROR gamma T and ROR alpha Inhibitor) VPR-66 inhibition of transcriptional activity determined in CHO cells transfected with RORa.

Formulation, Preparation, and Storage

Preparation Method

Please contact technical support for detailed reconstitution instructions.

Formulation

Crystalline, off-white solid; MW = 287.4

Concentration

Lyoph

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: VPR-66, ROR gamma T and ROR alpha Inhibitor

h17 cells are a sub-type of T-helper cells that secrete IL-17 and other pro-inflammatory cytokines. Th17 cells have been implicated in driving the pathophysiology of several human autoimmune diseases. Retinoic Acid-Related Orphan Nuclear, Receptors (ROR) are canonical nuclear receptors with transcriptional activity at specific DNA response elements. Although most nuclear receptors are regulated by ligand binding no cognate ligands(s) have been definitively identified for any ROR protein. There are three different ROR proteins: RORa, RORb and RORg. An isoform of RORg, called ROR gamma T, is exclusively expressed in lymphocytes. Together, RORa and ROR gamma T are critical transcription factors required for differentiation of naive CD4+ Tcells into Th17 cells.

Alternate Names

VPR 66, VPR66

Additional VPR-66, ROR gamma T and ROR alpha Inhibitor Products

Product Documents

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download NBP2-29335 - Protocol

COA

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Inhibitors are guaranteed for 1 year from date of receipt.

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