TLR (Cell Surface) Screening Antibody Pack

Flow Cytometry: TLR (Cell Surface) Screening Antibody Pack [NBP2-25086]

Flow Cytometry: TLR (Cell Surface) Screening Antibody Pack [NBP2-25086] - TLR1 and TLR2 expression pattern in primary BMNCs from 3 MM patients analyzed by flow cytometry. CD138-positive cells were gated from the total cell population. Staining for was compared with isotype-matched controls (NC). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY license. TLR1 Antibody [NB100-56563] and TLR2 Antibody (TL2.1) [NB100-56722] .

Dual RNAscope ISH-IHC: TLR (Cell Surface) Screening Antibody Pack [NBP2-25086] - Formalin-fixed paraffin-embedded tissue sections of human tonsil were probed for TLR4 mRNA (ACD RNAScope Probe, catalog #311281; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using Mouse Monoclonal (Novus Biologicals catalog #NB100-56566) at 5ug/mL with 1 hour incubation at room temperature followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes.

Western Blot: TLR (Cell Surface) Screening Antibody Pack [NBP2-25086]

Western Blot: TLR (Cell Surface) Screening Antibody Pack [NBP2-25086] - Expression of TLR2 was determined by western blotting. The immunoreactivity of the anti-TLR2 was confirmed with human intestinal lysate. Data are representative for analysis of >=2 independent experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY license. TLR2 Antibody (TL2.1) [NB100-56722]