PPAR delta/NR1C2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-962

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NB300-962
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Rat

Predicted:

Bovine (100%), Mouse (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Peptide ELISA, Immunoprecipitation

Cited:

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all PPAR delta/NR1C2 Antibodies »

Product Specifications

Immunogen

Peptide with sequence CHPLLQEIYKDMY corresponding to C-Terminus according to NP_006229.1.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 23555761).

Specificity

This antibody is expected to recognise isoform 1 (NP_006229) of PPAR delta, but not isoform 2, a shorter protein (NP_803184) with a different C-terminus.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PPAR delta/NR1C2 Antibody

Western Blot: PPAR delta/NR1C2 Antibody [NB300-962]

Western Blot: PPAR delta/NR1C2 Antibody [NB300-962]

Western Blot: PPAR delta/NR1C2 Antibody [NB300-962] - Staining of MOLT4 lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.
Western Blot: PPAR delta/NR1C2 Antibody [NB300-962]

Western Blot: PPAR delta/NR1C2 Antibody [NB300-962]

Western Blot: PPAR delta/NR1C2 Antibody [NB300-962] - PPAR delta in HeLa cell lysate (25 ug) using anti-PPAR delta antibody. Image from verified customer review.

Applications for PPAR delta/NR1C2 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

1 - 3 ug/mL
Application Notes
WB: Approx. 55 kDa band observed in human Lymphoblastoid and T-cell line MOLT4 lysates (calculated MW of 49.9 kDa band according to NP_006229.1). Use in immunoprecipitation reported in scientific literature (PMID: 23555761).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PPAR delta/NR1C2

This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) family. PPARs are nuclear hormone receptors that bind peroxisome proliferators and control the size and number of peroxisomes produced by cells. PPARs mediate a variety of biological processes, and may be involved in the development of several chronic diseases, including diabetes, obesity, atherosclerosis, and cancer. This protein is a potent inhibitor of ligand-induced transcription activity of PPAR alpha and PPAR gamma. It may function as an integrator of transcription repression and nuclear receptor signaling. The expression of this gene is found to be elevated in colorectal cancer cells. The elevated expression can be repressed by adenomatosis polyposis coli (APC), a tumor suppressor protein related to APC/beta-catenin signaling pathway. Knockout studies in mice suggested the role of this protein in myelination of the corpus callosum, lipid metabolism, and epidermal cell proliferation.

Long Name

Peroxisome Proliferator-activated Receptor delta

Alternate Names

NR1C2, NUC1, PPARD

Entrez Gene IDs

5467 (Human); 19015 (Mouse); 25682 (Rat)

Gene Symbol

PPARD

UniProt

Q03181 (Human)

Additional PPAR delta/NR1C2 Products

Product Documents for PPAR delta/NR1C2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PPAR delta/NR1C2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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