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PIDD Antibody (Anto-1) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97595

Novus Biologicals, part of Bio-Techne
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NBP1-97595

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # Anto-1

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Recombinant human PIDD death domain (aa 776-910).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse PIDD Antibody (Anto-1) - BSA Free (NBP1-97595) is a monoclonal antibody validated for use in IHC and WB. Anti-PIDD Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for PIDD Antibody (Anto-1) - BSA Free

Western Blot: PIDD Antibody (Anto-1)BSA Free [NBP1-97595]

Western Blot: PIDD Antibody (Anto-1)BSA Free [NBP1-97595]

Western Blot: LRDD Antibody (Anto-1) [NBP1-97595] - Analysis of endogenous and overexpressed human PIDD using MAb to PIDD (Anto-1). Method: Cell extracts (1x10^5) from HEK 293T wild type (lane 1) or transfected with a plasmid coding for human PIDD (lane 2) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with the MAb to PIDD (Anto-1) at a 1:500 dilution for 2 hours. Proteins were visualized using a peroxidase-conjugated polyclonal antibody to mouse IgG and a chemiluminescence detection system. Note that two major forms of PIDD are detected, representing full length and cleaved proteins, migrating at approximatively 90kDa and 50kDa, respectively.
Immunohistochemistry-Paraffin: PIDD Antibody (Anto-1) - BSA Free [NBP1-97595]

Immunohistochemistry-Paraffin: PIDD Antibody (Anto-1) - BSA Free [NBP1-97595]

Immunohistochemistry-Paraffin: LRDD Antibody (Anto-1) [NBP1-97595] - Staining of endogenous human PIDD in different human tissues (paraffin sections) using MAb to PIDD (Anto-1). Method: Different human normal (A: Bronchus; B: Kidney; C: Lung) or cancer (D: Breast; E: Ovarian; F: Colo-rectal) tissues were stained with MAb to PIDD (Anto-1) by standard immunohistochemistry.

Applications for PIDD Antibody (Anto-1) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Detects bands of ~90kDa (full length protein) and ~50kDa (cleaved protein) by Western blot.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PIDD

The protein encoded by the LRDD gene contains a leucine-rich repeat and a death domain. This protein has been shown to interact with other death domain proteins, such as Fas (TNFRSF6)-associated via death domain (FADD) and MAP-kinase activating death domain-containing protein (MADD), and thus may function as an adaptor protein in cell death-related signaling processes. The expression of the mouse counterpart of this gene has been found to be positively regulated by the tumor suppressor p53 and to induce cell apoptosis in response to DNA damage, which suggests a role for this gene as an effector of p53-dependent apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been reported. (provided by RefSeq)

Long Name

p53-Induced Protein With A Death Domain

Alternate Names

LRDD

Gene Symbol

PIDD1

Additional PIDD Products

Product Documents for PIDD Antibody (Anto-1) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PIDD Antibody (Anto-1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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