JunD Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38983

Novus Biologicals, part of Bio-Techne
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NBP2-38983
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all JunD Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: IIQSNGLVTTTPTSSQFLYPKVAASEEQEFAEGFVKALED

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit JunD Antibody - BSA Free (NBP2-38983) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for JunD Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: JunD Antibody [NBP2-38983]

Immunocytochemistry/ Immunofluorescence: JunD Antibody [NBP2-38983]

Immunocytochemistry/Immunofluorescence: JunD Antibody [NBP2-38983] - Staining of human cell line SH-SY5Y shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: JunD Antibody [NBP2-38983]

Immunohistochemistry-Paraffin: JunD Antibody [NBP2-38983]

Immunohistochemistry-Paraffin: JunD Antibody [NBP2-38983] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.
JunD Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: JunD Antibody - BSA Free [NBP2-38983]

Chromatin Immunoprecipitation-exo-Seq: JunD Antibody - BSA Free [NBP2-38983]

ChIP-Exo-Seq composite graph for Anti-JUND (NBP2-38983) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for JunD Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: JunD

The activator protein-1 (AP-1) transcription factor consists of either Jun/Jun homodimers or Fos/Jun heterodimeric complexes. Homo- and heterodimers bind to the TGACTCA consensus sequence present in numerous promoters and initially identified as the phorbol ester tumor promoter response element (TRE). Jun B and Jun D have been shown to be almost identical to c-Jun in their C-terminal regions, which are involved in dimerization and DNA binding, whereas their N-terminal domains, which are involved in transcriptional activation, diverge. All three form heterodimers among themselves and with c-Fos and other members of the Fos gene family. Studies suggest that the two forms of Jun D may be due to internal initiation of translation.

Long Name

JunD Proto-oncogene

Alternate Names

AP-1

Gene Symbol

JUND

UniProt

P17535

Additional JunD Products

Product Documents for JunD Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for JunD Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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