INSTA-Blot Rat Tissues
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-30112
Product Summary for INSTA-Blot Rat Tissues
Ready-to-use PVDF membrane with 11 rat tissue lysates (20 ug total protein per lane)
INSTA-Blot™is a ready-to-use PVDF membrane containing denatured protein from cells or tissue lysates. The INSTA-Blot™ Rat Tissues NBP2-30112 is a simple and fast solution for screening proteins from various rat tissues. We've streamlined the western blotting procedure by eliminating the sample aquisition, preparation, SDS-PAGE and electro-blotting steps. With INSTA-Blot™, immunoblotting is an easy six-step procedure: blocking, primary antibody incubation, wash, secondary antibody incubation, wash, and develop.
Product Specifications
Application Notes
Tissues are from Sprague Dawley rats. Tissues are lysed in lysis buffer (10 mM Tris, pH 8.0, 130 mM NaCl, 1% Triton X-100, 10mM NaF, 10 mM NaPi, 10 mM NaPPi) containing Protease Inhibitor Cocktail (PIC) and PMSF. Prior to SDS-PAGE the tissue lysate is resuspended 1:1 with 2X SDS sample buffer (120 mM Tris-Hcl [pH 6.8], 20 mM EDTA, 4% SDS, 0.06% Bromophenol Blue, 20% glycerol, 0.4% B-mercaptoethanol).
Scientific Data Images for INSTA-Blot Rat Tissues
![SDS-PAGE: INSTA-Blot Rat Tissues [NBP2-30112]](https://resources.bio-techne.com/images/products/INSTA-Blot-Rat-Tissues-SDS-Page-NBP2-30112-img0006.jpg "SDS-PAGE: INSTA-Blot Rat Tissues [NBP2-30112]")
SDS-PAGE: INSTA-Blot Rat Tissues [NBP2-30112]
SDS-Page: INSTA-Blot Rat Tissues [NBP2-30112] - Rat Tissues. Approximately 20 ug per lane of different rat tissue lysate are resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. Molecular weight marker; 2. Brain; 3. Heart; 4. Small Intestine; 5. Kidney; 6. Liver; 7. Lung; 8. Skeletal Muscle; 9. Stomach; 10. Spleen; 11. Ovary; 12. TestisFormulation, Preparation, and Storage
Preparation Method
INSTA-Blot™ Rat Tissues NBP2-30112 contains denatured proteins from rat lysates loaded at 20 ug (total protein, Bradford Assay) per lane on a 4-20% Tris-Glycine mini gel. After resolution by
SDS-PAGE at 125V for 1 hour the proteins are electro-blotted for 2 hours at 25V onto a PVDF membrane. The membrane is stained with amido black for visualization of proteins, dried and packaged under anoxic conditions. The INSTA-Blot™ is sold ready-to-use in your western blot protocol.
Concentration
Concentration is not relevant for this product. Please see the protocols for proper use of this product.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at room temperature.
Background: INSTA-Blot Rat Tissues
Alternate Names
INSTA Blot Rat Tissues, INSTA-Blot Rat, INSTABlot Rat Tissues
Additional INSTA-Blot Rat Tissues Products
Product Documents for INSTA-Blot Rat Tissues
Product Specific Notices for INSTA-Blot Rat Tissues
The molecular weights for the marker used with this product are: 180, 116, 97, 66, 44, 29, 18, 14, 6.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Western Blot Membranes are guaranteed for 1 year from date of receipt.
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