INSTA-Blot Human Cell Lines
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-30114
Key Product Details
Species
Human
Applications
Western Blot
Product Summary for INSTA-Blot Human Cell Lines
Mark12 is used as the molecular weight marker for this product.
INSTA-Blot͐ is a ready-to-use PVDF membrane containing denatured protein from cells or tissue lysates. The INSTA-Blot͐ Human Cell Lines IMB-105 is a simple and fast solution for screening proteins from various human cell lines. We've streamlined the western blotting procedure by eliminating the sample aquisition, preparation, SDS-PAGE and electro-blotting steps. With INSTA-Blot͐, immunoblotting is an easy six-step procedure: blocking, primary antibody incubation, wash, secondary antibody incubation, wash, and develop.
Product Specifications
Application Notes
Human cell lines are lysed in 1X lysis buffer (10 mM Tris, pH 8.0,130 mM NaCl, 1% Triton X-100, 10 mM NaF, 10 mM NaPi, 10mM NaPPi) containing Protease Inhibitor Cocktail (PIC) and PMSF. Prior to SDS-PAGE the cell lysate is resuspended 1:1 with 2X SDS sample buffer (120 mM Tris-Hcl [pH 6.8], 20 mM EDTA, 4% SDS, 0.06% Bromophenol Blue, 20% glycerol, 0.4% B-mercaptoethanol).Use in Western blot reported in scientific literature (PMID: 24842606)
Reactivity Notes
Human reactivity reported in scientific literature (PMID: 24842606)
Scientific Data Images for INSTA-Blot Human Cell Lines
![Western Blot: INSTA-Blot Human Cell Lines [NBP2-30114]](https://resources.bio-techne.com/images/products/INSTA-Blot-Human-Cell-Lines-SDS-Page-NBP2-30114-img0001.jpg "Western Blot: INSTA-Blot Human Cell Lines [NBP2-30114]")
Western Blot: INSTA-Blot Human Cell Lines [NBP2-30114]
Western Blot: INSTA-Blot Human Cell Lines [NBP2-30114] - Human Cell Lines. Approximately 20 ug per lane of human cell line lysates are resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. Molecular weight marker (Mark12); 2. HeLa; 3. Jurkat; 4. Daudi; 5. HEK 293; 6. Rh30; 7. A375; 8. T98G; 9. HCT-116; 10. Hep-2.Formulation, Preparation, and Storage
Preparation Method
INSTA-Blot͐ Human Cell Lines NBP2-30114 contains denatured proteins from human cell line lysates (See Cell Lysate Preparation) loaded at 20 ug (total protein, Bradford Assay) per lane on a 4-20% Tris-Glycine mini gel. After resolution by SDS-PAGE at 125V for 1 hour the proteins are electro-blotted for 2 hours at 25V onto a PVDF membrane. The membrane is stained with amido black for visualization of proteins, dried and packaged under anoxic conditions. The INSTA-Blot is sold ready-to-use in your western blot protocol.
Concentration
Concentration is not relevant for this product. Please see the protocols for proper use of this product.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at room temperature.
Background: INSTA-Blot Human Cell Lines
Alternate Names
INSTA Blot Human Cell Lines, INSTA-Blot Human, INSTABlot Human Cell Lines
Additional INSTA-Blot Human Cell Lines Products
Product Documents for INSTA-Blot Human Cell Lines
Product Specific Notices for INSTA-Blot Human Cell Lines
Mark12 is used as the molecular weight marker for this product.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Western Blot Membranes are guaranteed for 1 year from date of receipt.
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