UNveil Unstained Protein Ladder
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33175
Key Product Details
Applications
Western Blot
Product Summary for UNveil Unstained Protein Ladder
The Unveil Unstained Protein Ladder is a mixture of 12 unstained recombinant proteins covering a wide range of molecular weights from 10 to 200 kDa. The proteins resolve into clearly identifiable bands when separated on the SDS-PAGE gel (MES buffer), with the intensified 25kDa and 85kDa protein bands serving as the two reference bands. The Unveil Unstained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in the gel loading buffer and is ready to use.
(12 unstained bands, 10-200 kDa)
(12 unstained bands, 10-200 kDa)
Product Specifications
Application Notes
3 ul or 5 ul per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 ul per well for general Western transferring
Scientific Data Images for UNveil Unstained Protein Ladder
Western Blot: UNveil Unstained Protein Ladder [NBP3-33175] -
Western Blot: UNveil Unstained Protein Ladder [NBP3-33175] - The Unveil Unstained Protein Ladder is a mixture of 12 unstained recombinant proteins covering a wide range of molecular weights from 10 to 200 kDa. The proteins resolve into clearly identifiable bands when separated on the SDS-PAGE gel (MES buffer), with the intensified 25kDa and 85kDa protein bands serving as the two reference bands. The Unveil Unstained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in the gel loading buffer and is ready to use.Formulation, Preparation, and Storage
Formulation
20 mM Tris-phosphate (pH 7.5), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, 15 % (v/v) Glycerol.
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: UNveil Unstained Protein Ladder
Additional UNveil Unstained Protein Ladder Products
Product Documents for UNveil Unstained Protein Ladder
Product Specific Notices for UNveil Unstained Protein Ladder
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
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