TRIS base

Name: TRIS base
Brand: Tocris Bioscience
SKU: 3163
Rating: 5 (2 reviews)

Tocris Bioscience a Bio-Techne Brand

Catalog # 3163

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Key Product Details

Description:	Commonly used laboratory reagent; used to make TBS, TAE and TBE buffers
Chemical Name:	2-Amino-2-(hydroxymethyl)-1,3-propanediol
Purity:	≥99.5%
Molecular Weight:	121.14

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Biological Activity for TRIS base

TRIS base is a commonly used laboratory reagent. Highly water soluble. Working pH range 7 - 9. Used in buffer solutions such as Tris-buffered saline (TBS), Tris-acetate-EDTA (TAE) in combination with acetic acid and EDTA (Cat. No. 2811) and Tris-borate-EDTA in combination with boric acid and EDTA.

Technical Data for TRIS base

M. Wt	121.14
Formula	C₄H₁₁NO₃
Storage	Store at RT
Purity	≥99.5%
CAS Number	77-86-1
PubChem ID	6503
InChi Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Smiles	OCC(CO)(N)CO

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for TRIS base

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	water	363.42	3000

Preparing Stock Solutions for TRIS base

The following data is based on the product molecular weight 121.14.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
30 mM	0.28 mL	1.38 mL	2.75 mL
150 mM	0.06 mL	0.28 mL	0.55 mL
300 mM	0.03 mL	0.14 mL	0.28 mL
1500 mM	0.01 mL	0.03 mL	0.06 mL

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Reconstitution Calculator

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Citations for TRIS base

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Customer Reviews for TRIS base (2)

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Western blot

Name: Anonymous

Species: Other

Assay Type: In Vitro

We have used this product to make western blot gels of different percentages of acrylamide
Works as expected

Name: Anonymous

Species: Human

I used this for making TBS buffers for western blots. All seemed to work fine.

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Product Documents for TRIS base

COA

Certificate of Analysis

To download a Certificate of Analysis, please select a batch below.