Pepsin Solution

Novus Biologicals, part of Bio-Techne | Catalog # NB900-66724

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NB900-66724
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Key Product Details

Applications

Immunohistochemistry

Product Specifications

Specificity

Stablized Pepsin solution for antigen retrieval

Application Notes

Preparation of Working Solutions
1. Solution Pepsin is ready to use and does not require any preparation.

Protocol Recommendations
1. Deparaffinize tissue sections and wash slides with wash buffer.
2. Remove excess buffer from slide(s) without letting the tissue dry.
3. Depending on the size of the tissue section add 1-2 drops of pepsin solution.
4. Incubate slide(s) for 5-10 minutes at 37C.
5. Wash slide(s) with wash buffer and proceed with immunostaining.

Formulation, Preparation, and Storage

Preservative

No Preservative

Concentration

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C. Do not freeze.

Background: Pepsin Solution

Fixation is one of the most critical aspects of immunostaining. If the antigen is not properly fixed it will be washed out of the specimen. On the other hand, over fixation can cause severe problems such as masking or denaturation of the antigen. Formaldehyde is probably the best all-around fixative, due to its cross-linking characteristics. Length of fixation is very critical to prevent antigen masking. Immunoglobulins are especially susceptible to over fixation with formalin. In such cases, treatment with proteolytic enzyme is required to digest excess aldehyde linkages and to expose the antigen.

Additional Pepsin Solution Products

Product Documents for Pepsin Solution

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Pepsin Solution

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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