pCLNCX Retrovirus Expression Vector

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29502

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NBP2-29502
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Product Specifications

Applications

Retroviral Production (reported in scientific literature (PMID 29121057))

Application Notes

The RetroMax system is designed for maximal virus titer in 293 cells. It takes advantages of two properties of 293 cells, i) high level of transfectibility, ii) strong E1A-mediated stimulation of CMV promoter controlled transcription. 293 cells are of nonmurine origin, hence the problem of selective packaging and transfer of VL30 genomes (present in all murine packaging cells) are avoided. Vector supernatants are free of helper virus and are of sufficiently high titer within 2 days of transient transfection in 293 cells to permit infection of more than 50% of dividing target cells in culture.

Scientific Data Images for pCLNCX Retrovirus Expression Vector

pCLNCX Retrovirus Expression Vector [NBP2-29502] - Schematic presentation of pCLNCX vector: CMV immediate early promoter/enhancer allows highefficiency transcription in 293 cells. However, this is lost during viral replication. Hence, the gene of interest cloned into Eco RI cloning site is driven by Moloney MLV and murine sarcoma virus LTR (RU5). For expression under the control of CMV early promoter/enhancer, the gene of interest should be cloned into Hind III or Cla I site.

Formulation, Preparation, and Storage

Formulation

10 ug in 20 ul 1x TE (10 mM Tris, pH 7.5, 1 mM EDTA)

Concentration

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: pCLNCX Retrovirus Expression Vector

The pCLNCX expression vector is a part of the RetroMax expression system and has been designed to maximize recombinant-retrovirus titers in a simple, efficient, and flexible experimental system. All members of the RetroMax expression vector family (pCLXSN, pCLNCX, pCLNRX, and pCLNDX) have an extended packaging signal (omega+) and are derived from safetymodified retrovirus vectors in which the gag open reading frame has been stopped by a point mutation (1), thereby minimizing the opportunity for replication competent retrovirus production by recombination with packaging genome.

Alternate Names

pCLNCX, pCLNCX Retrovirus

Additional pCLNCX Retrovirus Expression Vector Products

Product Documents for pCLNCX Retrovirus Expression Vector

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Certificate of Analysis

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Product Specific Notices for pCLNCX Retrovirus Expression Vector

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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