I am having a retroviral system in place which allows me to infect mouse cell lines. The vectors I have are MIG.R (with my gene of interest), pCL-ECO and VSV-g. I know that my pCL-ECO vector is only suitable for infecting mouse cells. My question is: Is it possible to use my MIG.R and VSV-g vectors, and exchange the mouse specific pCL-ECO with pCL-Ampho or pCL-10A1 packaging vector which you both offer for purchase (Cat Numbers are 10045P, 10046P, 10047P). My aim is to infect human cell lines with my gene of interest.

These vectors, while also sold separately, are components of the RetroMax system 10040K. The vectors are designed to be used as described in the 10040K manual. Yes, you are correct, pCL-ECO is for mouse and rat, and not for human. We have not evaluated the Retromax system with the MIG.R and VSV-g vectors, and can not promise they would be compatible with your vectors. The Retromax packaging vectors are designed to be used with the expression vectors in the Retromax Kit. However, the packaging vectors are widely published and you may be able to find some information in the literature relevant to your model system (search scholar.google.com and keywords with Retromax and vector name, or keys words with packaging vector name and expression vector name ).

Can you please let me know if NBP2-29542 contain the Retrovirus ?

The pCL-10A1 Retrovirus Packaging Vector NBP2-29542 does not actually contain the retrovirus. The vector is just the DNA plasmid.

Can pCL-10A1 Retrovirus Packaging vector (NBP2-29542) be used with Lentivirus shRNA vector pGLV3?

When co-transfecting/transducing pCL-10A1 with a pCLX/N expression vector in the acell line, pCL-10A1 will produce retrovial Gag, Pol, and Env proteins for packing the pCLX/N-expressed viral RNA genome. [pCLX/N expression vectors have both Amp and Neo genes for growing in E.coli and stably transfection in a mammalian cell line, respectively] A key feature for appropriate packaging into viral protein shells is the packaging signal(s) UR5 on the viral RNA genome, which is derived from one of pCLX/N expression vectors. Check the nucleotide sequence(s) for the packaging signal(s) on your plasmid and compare to established UR5 sequence(s) to asses the likelihood of co-transduction experiments using pCL-10A1 and your vector successfully producing infectious virions.

pCL-10A1 Retrovirus Packaging Vector

Name: pCL-10A1 Retrovirus Packaging Vector
Brand: Novus Biologicals
SKU: NBP2-29542

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29542

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Product Specifications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

Mouse, Mammal

Applications

N/A

Product Specifications

Specificity

Ecotropic (MoMuLV) for mouse and rat cells only (not human)
Amphotropic (from 4070A MuLV) for most mammalian cells (but not hamster)
10A1 (MuLV) for most mammalian cells (including hamster)

Application Notes

AMPLIFICATION: The plasmid DNA contains an ampicillin resistant gene. For large scale production, this plasmid may be amplified on LB plates containing 50 ug/ml ampicillin. The liquid cultures may be grown at 100 ug ampicillin/ml. Use in retroviral production reported in multiple pieces of scientific literature.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:33168978) Human reactivity reported in scientific literature (PMID: 23973628)

Formulation, Preparation, and Storage

Formulation

10 ug in 1x TE (10 mM Tris, pH 7.5, 1 mM EDTA)

Concentration

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: pCL-10A1 Retrovirus Packaging Vector

The pCL-10A1 packaging vector is a part of the RetroMax expression system and has been designed to maximize recombinant-retrovirus titers in a simple, efficient, and flexible experimental system. By introducing a retroviral vector into a cell expressing retroviral proteins, retroviral particles (virions) are shed into the culture medium at the rate of at the rate of about 1 infectious particle/cell/day Retrovirus tropism is determined at 3 levels. The first is simply a function of viral envelope protein, gp70. The envelope determines which cells the virus will enter. pCL-10A1 expresses 10A1 envelope protein that was isolated from a mouse infected with amphotropic MuLV (2,3). Retroviruses obtained by cotransfection with pCL-10A1 vector will infect most mammalian cells including hamster cells.

Alternate Names

pcl 10a1 retrovirus packaging vector, pCL10A1, pCL-10A1, pCL-10A1 Retrovirus

Additional pCL-10A1 Retrovirus Packaging Vector Products

Product Documents for pCL-10A1 Retrovirus Packaging Vector

Product Datasheets

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Protocols & Manuals

Download Background and Contents -pCL-10A1 Retrovirus Packaging Vector

Download Manual - Retroviral Expression System

Download NBP2-29542 - Protocol

Download Product Handling Protocol - pCL-10A1 Retrovirus Packaging Vector

Download Protocol - pCL-10A1 Retrovirus Packaging Vector

COA

Product Specific Notices for pCL-10A1 Retrovirus Packaging Vector

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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