L-Azidonorleucine hydrochloride
Tocris Bioscience, part of Bio-Techne | Catalog # 6585
Unnatural amino acid for bio-orthogonal or fluorescent labeling of newly synthesized proteins
Key Product Details
Description
Unnatural amino acid for bio-orthogonal or fluorescent labeling of newly synthesized proteins
Product Description
L-Azidonorleucine hydrochloride is an unnatural amino acid; methionine surrogate. Can be incorporated into proteins by a mutant methionyl tRNA synthetase to enable cell-type specific analysis of protein synthesis. For use with bio-orthogonal non-canonical amino-acid tagging (BONCAT) or fluorescent non-canonical amino acid tagging (FUNCAT). Labeling does not affect the normal localization of the labeled proteins.Product Specifications
Molecular Weight
208.65
Formula
C6H12N4O2.HCl
Storage
Store at -20°C
Chemical Name
(S)-2-Amino-6-azidohexanoic acid hydrochloride
CAS Number
1454334-76-9
PubChem ID
121235322
InChI Key
RCEAACZNVVRXSJ-JEDNCBNOSA-N
SMILES
N[C@H](C(O)=O)CCCCN=[N+]=[N-].Cl
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 20.86 | 100 |
Preparing Stock Solutions
for L-Azidonorleucine hydrochloride
The following data is based on the product molecular weight 208.65
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 4.79 mL | 23.96 mL | 47.93 mL |
| 5 mM | 0.96 mL | 4.79 mL | 9.59 mL |
| 10 mM | 0.48 mL | 2.40 mL | 4.79 mL |
| 50 mM | 0.10 mL | 0.48 mL | 0.96 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Mahdavi Engineered aminoacyl-tRNA synthetase for cell-selective analysis of mammalian protein synthesis. J.Am.Chem.Soc. 2016 PMID: 26991063
- Alvarez-Castelao Cell-type-specific metabolic labeling of nascent proteomes in vivo. Nat.Biotechnol. 2017 PMID: 29106408
Product Specific Notices for L-Azidonorleucine hydrochloride
For research use only
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