L-Azidohomoalanine hydrochloride

Product Description

L-Azidohomoalanine hydrochloride is an unnatural amino acid. Incorporated into proteins during de novo protein synthesis, with no effect on protein degradation. Requires methionine-free medium for efficient incorporation. Substrate for wild-type methionyl-tRNA synthetase (MetRS) in vivo. Can be used in BONCAT (bio-orthogonal non-canonical amino acid tagging) for the identification of newly synthesized proteins.

Solubility

Preparing Stock Solutions for L-Azidohomoalanine hydrochloride

The following data is based on the product molecular weight 180.59

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	5.54 mL	27.69 mL	55.37 mL
5 mM	1.11 mL	5.54 mL	11.07 mL
10 mM	0.55 mL	2.77 mL	5.54 mL
50 mM	0.11 mL	0.55 mL	1.11 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Lang & Chin Cellular incorporation of unnatural amino acids and bioorthogonal labeling of proteins. Chem.Rev. 2014 PMID: 24655057
• Wang Nonradioactive quantification of autophagic protein degradation with L-azidohomoalanine labeling. Nat.Protoc. 2017 PMID: 28079880
• Dieterich Selective identification of newly synthesized proteins in mammalian cells using bioorthogonal noncanonical amino acid tagging (BONCAT). Proc.Natl.Acad.Sci.U.S.A. 2006 PMID: 16769897