IRIS11 Prestained Protein Ladder
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33168
Key Product Details
Applications
Western Blot
Product Summary for IRIS11 Prestained Protein Ladder
The IRIS11 Prestained Protein Ladder is a combination of 11 pre-stained proteins with molecular weights from 3 to 260 kDa. The 11 recombinant proteins are covalently coupled with blue chromophore, while 2 red bands at 70 kDa and 260 kDa, a green band at 15 kDa and a newly designed peacock green band at 60 kDa serve as reference bands. The IRIS11 Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating the target protein size and validating the Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.
(11 pre-stained bands, 3-260 kDa)
(11 pre-stained bands, 3-260 kDa)
Product Specifications
Application Notes
3ul or 5ul per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5ul per well for general Western transferring.
Scientific Data Images for IRIS11 Prestained Protein Ladder
Western Blot: IRIS11 Prestained Protein Ladder [NBP3-33168] -
Western Blot: IRIS11 Prestained Protein Ladder [NBP3-33168] - The IRIS11 Prestained Protein Ladder is a combination of 11 pre-stained proteins with molecular weights from 3 to 260 kDa. The 11 recombinant proteins are covalently coupled with blue chromophore, while 2 red bands at 70 kDa and 260 kDa, a green band at 15 kDa and a newly designed peacock green band at 60 kDa serve as reference bands. The IRIS11 Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating the target protein size and validating the Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.Formulation, Preparation, and Storage
Formulation
20 mM Trisphosphate at pH 7.5, 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol.
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
Background: IRIS11 Prestained Protein Ladder
Additional IRIS11 Prestained Protein Ladder Products
Product Documents for IRIS11 Prestained Protein Ladder
Product Specific Notices for IRIS11 Prestained Protein Ladder
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
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