INSTA-Blot Mouse Brain
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-30120
Product Summary for INSTA-Blot Mouse Brain
Ready-to-use PVDF membrane with mouse brain lysate (30 ug total protein per lane).
INSTA-Blot™ is a ready-to-use PVDF membrane containing denatured protein from cells or tissue lysates. The INSTA-Blot™ Mouse Brain NBP2-30120 is a simple and fast solution for screening proteins from various human, mouse and rat tissues. We've streamlined the western blotting procedure by eliminating the sample aquisition, preparation, SDS-PAGE and electro-blotting steps. With INSTA-Blot™, immunoblotting is an easy six-step procedure: blocking, primary antibody incubation, wash, secondary antibody incubation, wash, and develop.
Product Specifications
Application Notes
Mouse tissues are from Balb/c mice. Tissues are lysed in lysis buffer (10 mM Tris, pH 8.0, 130 mM NaCl, 1% Triton X-100, 10 mM NaF, 10 mM NaPi, 10 mM NaPPi) containing Protease Inhibitor Cocktail (PIC) and PMSF. Prior to SDS-PAGE the tissue lysate is resuspended 1:1 with 2X SDS sample buffer (120 mM Tris-Hcl [pH 6.8], 20 mM EDTA, 4% SDS, 0.06% Bromophenol Blue, 20% glycerol, 0.4% B-mercaptoethanol).
Scientific Data Images for INSTA-Blot Mouse Brain
![SDS-PAGE: INSTA-Blot Mouse Brain [NBP2-30120]](https://resources.bio-techne.com/images/products/INSTA-Blot-Mouse-Brain-SDS-Page-NBP2-30120-img0001.jpg "SDS-PAGE: INSTA-Blot Mouse Brain [NBP2-30120]")
SDS-PAGE: INSTA-Blot Mouse Brain [NBP2-30120]
SDS-Page: INSTA-Blot Mouse Brain [NBP2-30120] - Approximately 30 ug per lane of tissue lysate is resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. Molecular weight marker; 2-12 mouse brain.Formulation, Preparation, and Storage
Preparation Method
The INSTA-Blot™ Mouse Brain NBP2-30120 contains denatured proteins from tissue lysates loaded at 30 ug (total protein, Bradford Assay) per lane on a 4-20% Tris-Glycine mini gel. After resolution by SDS-PAGE at 125V for 1 hour the proteins are electro-blotted for 2 hours at 25V onto a PVDF membrane. The membrane is stained with amido black for visualization of proteins, dried and packaged under anoxic conditions. The INSTA-Blot™ is provided ready-to-use in your western blot protocol.
Concentration
Concentration is not relevant for this product. Please see the protocols for proper use of this product.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at room temperature.
Background: INSTA-Blot Mouse Brain
Alternate Names
INSTA Blot Mouse Brain, INSTABlot Mouse Brain
Additional INSTA-Blot Mouse Brain Products
Product Documents for INSTA-Blot Mouse Brain
Product Specific Notices for INSTA-Blot Mouse Brain
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Western Blot Membranes are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...