IA-Alkyne

Name: IA-Alkyne
Brand: Tocris Bioscience
SKU: 7015

Tocris Bioscience, part of Bio-Techne | Catalog # 7015

Citations (1)

COA / SDS

Broad spectrum cysteine reactive probe

Key Product Details

Description:	Broad spectrum cysteine reactive probe
Chemical Name:	N-5-Hexyn-1-yl-2-iodoacetamide
Purity:	≥97%
Molecular Weight:	265.09

View Full Technical Details for IA-Alkyne

Technical Data
Citations

Biological Activity for IA-Alkyne

IA-Alkyne is a broad spectrum cysteine reactive probe. Covalently binds cysteine residues in proteins. Can be utilized for covalent fragment-based ligand discovery (FBLD) and to identify Zn²⁺-binding cysteines. Used as a click-chemistry handle to attach a fluorophore or biotin to reactive cysteines in proteins for visualization by in-gel fluorescence or identification by mass spectrometry, respectively. Covalently binds cysteine residues in and activates TRPA1 channels.

Technical Data for IA-Alkyne

M. Wt	265.09
Formula	C₈H₁₂INO
Storage	Store at -20°C
Purity	≥97%
CAS Number	930800-38-7
PubChem ID	44448710
InChi Key	YCRUVTMZPHEOAM-UHFFFAOYSA-N
Smiles	ICC(=O)NCCCCC#C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for IA-Alkyne

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	26.51	100
	ethanol	26.51	100

Preparing Stock Solutions for IA-Alkyne

The following data is based on the product molecular weight 265.09.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.77 mL	18.86 mL	37.72 mL
5 mM	0.75 mL	3.77 mL	7.54 mL
10 mM	0.38 mL	1.89 mL	3.77 mL
50 mM	0.08 mL	0.38 mL	0.75 mL

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Reconstitution Calculator

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References for IA-Alkyne

References are publications that support the biological activity of the product.

Pace and Weerapana A competitive chemical-proteomic platform to identify zinc-binding cysteines. ACS Chem.Biol. 2014 PMID: 24111988
Macpherson Noxious compounds activate TRPA1 ion channels through covalent modification of cysteines. Nature 2007 PMID: 17237762
Backus Proteome-wide covalent ligand discovery in native biological systems. Nature 2016 PMID: 27309814