What is the binding capacity of the ATP Agarose?

Name: g-linked ATP agarose resin
Brand: Novus Biologicals
SKU: 510-0002

The binding of any ATP-binding protein to our resin will depend on a number of factors. These include amongst others: the formulation of the binding buffer, the incubation time of the sample with the resin (flow rate), the concentration of the ATP-binding protein, the affinity of the binding protein for ATP and the size of the binding protein (steric hindrance can be a factor with larger proteins). The only true way to establish the capacity of the column for any individual binding protein is by experimentation. If you require mg scale purification then our ATP agarose should be suitable, especially as the resin can be reused. The unique nature of our ATP resin is that it is not subject to dephosphorylation like many other similar resins available from other suppliers. This means that you can simply wash, re-equilibrate and reuse the resin multiple times. This will enable you to obtain mg quantities of your protein even if it takes more than one purification.

g-linked ATP agarose resin

Novus Biologicals, part of Bio-Techne | Catalog # 510-0002

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Product Summary
Product Specifications
Preparation & Storage
Background
Product Documents
Specific Notices

Product Summary for g-linked ATP agarose resin

Affinity resins have been widely used for the purification of enzymes that bind nucleotides and related molecules. Resins in which ATP is linked via the gamma-phosphate have been valuable in identifying proteins in the purine-binding proteome, which includes kinases, heat shock proteins and other ATP-binding proteins.

ATP Agarose resin comprises ATP attached to agarose beads via its gamma-phosphate. Two forms of the resin are available with low and high ligand substitution. A long hydrophilic spacer (14-atom) is used to minimise unwanted hydrophobic interactions and to facilitate unhindered interactions with biomolecules.

The Control Resin is just the agarose with no ATP bound.

Product Specifications

Specificity

Application Notes

High (8-12 umol/ml) - 2ml

Formulation, Preparation, and Storage

Concentration

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C. Do not freeze.

Background: g-linked ATP agarose resin

ATP linked to a support via its g-phosphate has been used for affinity purification of kinases, heat-shock proteins and other ATP-binding proteins. KinaseBind is a g-phosphate-linked ATP resin with a long hydrophillic spacer that facilitates molecular interactions with biomolecules. Linkage of ATP via the terminal phosphate means that the immobilised ligand cannot be removed by phosphatases in crude extracts.

Additional g-linked ATP agarose resin Products

Product Documents for g-linked ATP agarose resin

Product Datasheets

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Protocols & Manuals

Download Manual - g-linked ATP agarose resin

COA

Product Specific Notices for g-linked ATP agarose resin

This product is manufactured by Abcam and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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