Isolation involves ultracentrifugation and microfiltration. Exosomes are quantified and validated for protein content and particle number by Nanoparticles Tracking Analysis. Lyophilization does not alter stability of exosome proteins and nucleic acids.
Lyophilized from cell culture media
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.
Add deionized water, 100 ul for Standard 100 ug and 30 ul for Standard 30 ug, to get a final concentration of 1 mg/mL. Resuspend exosomes pipetting the solution up and down 10-15 times, avoiding bubbles. Vortex the reconstituted standard for 60 seconds.
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Store at 4C. After reconstitution store at -70C.