DL-Dithiothreitol
Tocris Bioscience, part of Bio-Techne | Catalog # 3154
Maintains SH groups in the reduced state
Key Product Details
Description
Maintains SH groups in the reduced state
Alternative Names
DTT,Cleland's reagent,Sputolysin
Product Description
DL-Dithiothreitol is a reagent for maintaining SH groups in the reduced state.Product Specifications
Molecular Weight
154.25
Formula
C4H10O2S2
Storage
Store at +4°C
Purity
≥98%
Chemical Name
threo-1,4-Dimercapto-2,3-butanediol
CAS Number
3483-12-3
PubChem ID
51341375
InChI Key
DCRJBUYBNCQKEE-CKQOXSRLSA-N
SMILES
SC[C@H](O)[C@@H](O)CS.SC[C@@H](O)[C@H](O)CS
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 15.42 | 100 |
Preparing Stock Solutions
for DL-Dithiothreitol
The following data is based on the product molecular weight 154.25
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 6.48 mL | 32.41 mL | 64.83 mL |
| 5 mM | 1.30 mL | 6.48 mL | 12.97 mL |
| 10 mM | 0.65 mL | 3.24 mL | 6.48 mL |
| 50 mM | 0.13 mL | 0.65 mL | 1.30 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Cleland Dithiothreitol, a new protective group for SH groups. Biochemistry 1964 PMID: 14192894
- Sears Relative susceptibilities of the interchain disulfides of an immunoglobulin G molecule to reduction by dithiothreitol. Biochemistry 1977 PMID: 403938
- Jabusch The effect of thiodiglycol and dithiothreitol on the alkaline hydrolysis products of certain amino acid phenylthiohydantoins. Anal.Biochem. 1978 PMID: 9762140
- Rüegg and Rudinger Reductive cleavage of cystine disulfides with tributylphosphine. Methods Enzymol. 1977 PMID: 927167
Product Specific Notices for DL-Dithiothreitol
For research use only
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