DL-Dithiothreitol

Name: DL-Dithiothreitol
Brand: Tocris Bioscience
SKU: 3154

Tocris Bioscience a Bio-Techne Brand

Catalog # 3154

Citations (1)

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Key Product Details

Description:	Maintains SH groups in the reduced state
Alternative Names:	DTT, Cleland's reagent, Sputolysin
Chemical Name:	threo-1,4-Dimercapto-2,3-butanediol
Purity:	≥98%
Molecular Weight:	154.25

View Full Technical Details for DL-Dithiothreitol

Biological Activity for DL-Dithiothreitol

DL-Dithiothreitol is a reagent for maintaining SH groups in the reduced state.

Technical Data for DL-Dithiothreitol

M. Wt	154.25
Formula	C₄H₁₀O₂S₂
Storage	Store at +4°C
Purity	≥98%
CAS Number	3483-12-3
PubChem ID	51341375
InChi Key	DCRJBUYBNCQKEE-CKQOXSRLSA-N
Smiles	SC[C@H](O)[C@@H](O)CS.SC[C@@H](O)[C@H](O)CS

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for DL-Dithiothreitol

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	water	15.42	100

Preparing Stock Solutions for DL-Dithiothreitol

The following data is based on the product molecular weight 154.25.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	6.48 mL	32.41 mL	64.83 mL
5 mM	1.30 mL	6.48 mL	12.97 mL
10 mM	0.65 mL	3.24 mL	6.48 mL
50 mM	0.13 mL	0.65 mL	1.30 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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References for DL-Dithiothreitol

References are publications that support the biological activity of the product.

Jabusch The effect of thiodiglycol and dithiothreitol on the alkaline hydrolysis products of certain amino acid phenylthiohydantoins. Anal.Biochem. 1978 PMID: 9762140
Rüegg and Rudinger Reductive cleavage of cystine disulfides with tributylphosphine. Methods Enzymol. 1977 PMID: 927167
Sears Relative susceptibilities of the interchain disulfides of an immunoglobulin G molecule to reduction by dithiothreitol. Biochemistry 1977 PMID: 403938
Cleland Dithiothreitol, a new protective group for SH groups. Biochemistry 1964 PMID: 14192894

Citations for DL-Dithiothreitol (1)

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Citations are publications that use Bio-Techne products. Selected citations for DL-Dithiothreitol include:

Baca et al. (2013) Gene-environment interactions affect long-term depression (LTD) through changes in DA receptor affinity in Snap25 deficient mice. Brain Res. 1532:85
PMID: 23939223

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