CD9 Immunobeads for Exosome Isolation (Mouse)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14799

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NBP3-14799
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Key Product Details

Species

Human

Applications

ELISA

Product Summary for CD9 Immunobeads for Exosome Isolation (Mouse)

CD9 Immunocapture Beads are 0.4 um latex beads covalently coupled with antibodies against extracellular vesicle (EV) surface antigen CD9. Samples containing EVs are incubated with Immunocapture Beads for rapid and specific isolation of EVs expressing CD9.

Using the supplied Elution Buffer, EVs can be eluted from Immunocapture beads and used in downstream analyses such as protein profiling and nucleic acid extraction.

Immunobeads can be re-used after incubation with Bead Regeneration Buffer.

Shelf life of 8 months.

Product Specifications

Sample Volume

0.5 - 1 mL

Scientific Data Images for CD9 Immunobeads for Exosome Isolation (Mouse)

CD9 Immunobeads for Exosome Isolation (Mouse)

CD9 Immunobeads for Exosome Isolation (Mouse) [NBP3-14799]

CD9 Immunobeads for Exosome Isolation (Mouse) [NBP3-14799] - Alix expression by western blotting of human plasma exosomes captured on lmmunobeads from 0.5 ml of plasma in comparison with exosomes purified via ultracentrifuge. 1, 3 and 5: lmmunocapture with immunobeads from 1 ml, 0.5 ml and 100 µ1 of human plasma respectively. 2, 4, 6: Ultra centrifuged exosomes from plasma after immunobeads capture. 7 lmmunocapture with immunobeads isotype coupled. 8, 9: Ultracentifuged exosome from 0.5 ml and 1 ml of human plasma.

Kit Contents for CD9 Immunobeads for Exosome Isolation (Mouse)

  • Bead Regeneration Buffer
  • CD9 Immunocapture Beads
  • Exosome Elution Buffer

Formulation, Preparation, and Storage

Concentration

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C.

Background: CD9 Immunobeads for Exosome Isolation (Mouse)

Immunocapture for isolation of exosomes relies on the antigen-antibody interaction. Antibodies directed at target of interest (eg CD9, CD63) are coated either in a 96-well plate or conjugated to a bead. The sample is applied directly into coated well, or into sample with coated beads, and EVs are allowed to bind. In a final step, EVs can be eluted from the antibody and used in downstream functional and phenotypic analysis. This method of isolation is highly specific, resulting in extremely pure EV populations.

Alternate Names

EV isolation, exosome isolation, extracellular vesicle isolation

Additional CD9 Immunobeads for Exosome Isolation (Mouse) Products

Product Documents for CD9 Immunobeads for Exosome Isolation (Mouse)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CD9 Immunobeads for Exosome Isolation (Mouse)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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