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Blu13 Prestained Protein Ladder

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33174

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NBP3-33174

Key Product Details

Applications

Western Blot

Product Summary for Blu13 Prestained Protein Ladder

The Blu13 Prestained Protein Ladder is a combination of 13 pre-stained proteins with molecular weights from 5 to 245 kDa. The 13 recombinant proteins are covalently coupled with blue chromophore, while 1 green band at 25 kDa and a red band at 75 kDa serve as reference bands. The Blu13 Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating protein size and validating Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.

(13 pre-stained bands, 5-245 kDa)

Product Specifications

Application Notes

3 ul or 5 ul per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 ul per well for general Western transferring.

Scientific Data Images for Blu13 Prestained Protein Ladder

Blu13 Prestained Protein Ladder

Western Blot: Blu13 Prestained Protein Ladder [NBP3-33174] -

Western Blot: Blu13 Prestained Protein Ladder [NBP3-33174] - The Blu13 Prestained Protein Ladder is a combination of 13 pre-stained proteins with molecular weights from 5 to 245 kDa. The 13 recombinant proteins are covalently coupled with blue chromophore, while 1 green band at 25 kDa and a red band at 75 kDa serve as reference bands. The Blu13 Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating protein size and validating Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.

Formulation, Preparation, and Storage

Formulation

20 mM Trisphosphate pH 7.5 at 25C, 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, 15% (v/v) Glycerol.

Concentration

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Blu13 Prestained Protein Ladder

Additional Blu13 Prestained Protein Ladder Products

Product Documents for Blu13 Prestained Protein Ladder

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Blu13 Prestained Protein Ladder

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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