Blu12 Prestained Protein Ladder
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33173
Key Product Details
Applications
Western Blot
Product Summary for Blu12 Prestained Protein Ladder
The Blu12 Prestained Protein Ladder is a combination of 12 pre-stained proteins with molecular weights from 11 to 245 kDa. The 12 recombinant proteins are covalently coupled with blue chromophore, while 1 green band at 25 kDa and a red band at 75 kDa serve as reference bands. The Blu12 Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating protein size and validating Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.
(12 pre-stained bands, 11-245 kDa)
(12 pre-stained bands, 11-245 kDa)
Product Specifications
Application Notes
3 ul or 5 ul per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 ul per well for general Western transferring.
Scientific Data Images for Blu12 Prestained Protein Ladder
Western Blot: Blu12 Prestained Protein Ladder [NBP3-33173] -
Western Blot: Blu12 Prestained Protein Ladder [NBP3-33173] - The Blu12 Prestained Protein Ladder is a combination of 12 pre-stained proteins with molecular weights from 11 to 245 kDa. The 12 recombinant proteins are covalently coupled with blue chromophore, while 1 green band at 25 kDa and a red band at 75 kDa serve as reference bands. The Blu12 Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating protein size and validating Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.Formulation, Preparation, and Storage
Formulation
20 mM Tris-phosphate (pH 7.5), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, 15 % (v/v) Glycerol.
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Blu12 Prestained Protein Ladder
Additional Blu12 Prestained Protein Ladder Products
Product Documents for Blu12 Prestained Protein Ladder
Product Specific Notices for Blu12 Prestained Protein Ladder
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
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