Biotin-PEG3-Azide
Tocris Bioscience, part of Bio-Techne | Catalog # 7524
Biotin with PEG linker and reactive click handle
Key Product Details
Description
Biotin with PEG linker and reactive click handle
Product Description
Biotin-PEG3-Azide is a biotinylation reagent for labeling alkyne-containing biomolecules either via Cu(I)-catalyzed Azide-Alkyne Click Chemistry reaction (CuAAC) or via Cu(I)-free Strain-Promoted Alkyne-Azide Click Chemistry (SPAAC) reaction with cyclooctyne derivatives.Product Specifications
Molecular Weight
444.55
Formula
C18H32N6O5S
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
(3aS,4S,6aR)-N-[2-[2-[2-(2-Azidoethoxy)ethoxy]ethoxy]ethyl]hexahydro-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanamide
CAS Number
875770-34-6
PubChem ID
60146223
InChI Key
ZWFOOMQCYIGZBE-ZOBUZTSGSA-N
SMILES
C(CCCC(NCCOCCOCCOCCN=[N+]=[N-])=O)[C@H]1[C@@]2([C@](CS1)(NC(=O)N2)[H])[H]
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 44.45 | 100 | |
| DMSO | 44.45 | 100 |
Preparing Stock Solutions
for Biotin-PEG3-Azide
The following data is based on the product molecular weight 444.55
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.25 mL | 11.25 mL | 22.49 mL |
| 5 mM | 0.45 mL | 2.25 mL | 4.50 mL |
| 10 mM | 0.22 mL | 1.12 mL | 2.25 mL |
| 50 mM | 0.04 mL | 0.22 mL | 0.45 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Zhang Use of NAD tagSeq II to identify growth phase-dependent alterations in E. coli RNA NAD+ capping. Proc.Natl.Acad.Sci.U.S.A. 2021 PMID: 33782135
Product Specific Notices for Biotin-PEG3-Azide
For research use only
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