Biocytin
Tocris Bioscience, part of Bio-Techne | Catalog # 3349
Versatile marker for neuroanatomical investigations
Key Product Details
Description
Versatile marker for neuroanatomical investigations
Product Description
Biocytin is a versatile marker used in anterograde, retrograde and intracellular neuroanatomical investigations and in biotinidase assays. Displays high solubility in aqueous solutions and has a low molecular weight facilitating injection using micropipettes. Can be incorporated with a variety of avidin and streptavidin conjugates for detection by light, fluorescence or electron microscope.Product Specifications
Molecular Weight
372.48
Formula
C16H28N4O4S
Storage
Store at -20°C
Chemical Name
N6-[5-[(3aS,4S,6aR)-Hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]-L-lysine
CAS Number
576-19-2
PubChem ID
83814
InChI Key
BAQMYDQNMFBZNA-MNXVOIDGSA-N
SMILES
O=C1N[C@@]2([H])[C@@](CS[C@H]2CCCCC(NCCCC[C@H](N)C(O)=O)=O)([H])N1
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 18.62 | 50 with gentle warming |
Preparing Stock Solutions
for Biocytin
The following data is based on the product molecular weight 372.48
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.5 mM | 5.37 mL | 26.85 mL | 53.69 mL |
| 2.5 mM | 1.07 mL | 5.37 mL | 10.74 mL |
| 5 mM | 0.54 mL | 2.68 mL | 5.37 mL |
| 25 mM | 0.11 mL | 0.54 mL | 1.07 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Roth Morphology and axonal projection pattern of neurons in the telencephalon of the fire-bellied toad Bombina orientalis: an anterograde, retrograde and intracellular biocytin labelling study. J.Comp.Neurol. 2004 PMID: 15334648
- MacNeil and Gaul Biocytin wide-field bipolar cells in rabbit retina selectively contact blue cones. J.Comp.Neurol. 2008 PMID: 17990268
- Kobbert Current concepts in neuroanatomical tracing. Prog.Neurobiol. 2000 PMID: 10856608
Product Specific Notices for Biocytin
For research use only
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