Biocytin

Name: Biocytin
Brand: Tocris Bioscience
SKU: 3349

Tocris Bioscience a Bio-Techne Brand

Catalog # 3349

Citations (12)

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Key Product Details

Description:	Versatile marker for neuroanatomical investigations
Chemical Name:	N⁶-[5-[(3aS,4S,6aR)-Hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]-L-lysine
Molecular Weight:	372.48

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Biological Activity for Biocytin

Biocytin is a versatile marker used in anterograde, retrograde and intracellular neuroanatomical investigations and in biotinidase assays. Displays high solubility in aqueous solutions and has a low molecular weight facilitating injection using micropipettes. Can be incorporated with a variety of avidin and streptavidin conjugates for detection by light, fluorescence or electron microscope.

Technical Data for Biocytin

M. Wt	372.48
Formula	C₁₆H₂₈N₄O₄S
Storage	Store at -20°C
CAS Number	576-19-2
PubChem ID	83814
InChi Key	BAQMYDQNMFBZNA-MNXVOIDGSA-N
Smiles	O=C1N[C@@]2([H])[C@@](CS[C@H]2CCCCC(NCCCC[C@H](N)C(O)=O)=O)([H])N1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Biocytin

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	water	18.62	50 with gentle warming

Preparing Stock Solutions for Biocytin

The following data is based on the product molecular weight 372.48.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	5.37 mL	26.85 mL	53.69 mL
2.5 mM	1.07 mL	5.37 mL	10.74 mL
5 mM	0.54 mL	2.68 mL	5.37 mL
25 mM	0.11 mL	0.54 mL	1.07 mL

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References for Biocytin

References are publications that support the biological activity of the product.

MacNeil and Gaul Biocytin wide-field bipolar cells in rabbit retina selectively contact blue cones. J.Comp.Neurol. 2008 PMID: 17990268
Roth Morphology and axonal projection pattern of neurons in the telencephalon of the fire-bellied toad Bombina orientalis: an anterograde, retrograde and intracellular biocytin labelling study. J.Comp.Neurol. 2004 PMID: 15334648
Kobbert Current concepts in neuroanatomical tracing. Prog.Neurobiol. 2000 PMID: 10856608

Citations for Biocytin (12)

Showing 1 - 10 of 12 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Biocytin include:

Roesler et al. (2019) Myosin XVI Regulates Actin Cytoskeleton Dynamics in Dendritic Spines of Purkinje Cells and Affects Presynaptic Organization. Front Cell Neurosci. 13:330
PMID: 31474830
Ferrarese et al. (2018) Dendrite-Specific Amplification of Weak Synaptic Input during Network Activity In Vivo. Cell Rep. 24:3455
PMID: 30257207
Estakhr et al. (2017) Differential Control of Dopaminergic Excitability and Locomotion by Cholinergic Inputs in Mouse Substantia Nigra. Curr Biol. 27:1900
PMID: 28648825
Kim and Cho (2017) Encoding of Discriminative Fear Memory by Input-Specific LTP in the Amygdala. Neuron. 95:1129
PMID: 28823727
Chai et al. (2017) Neural Circuit-Specialized Astrocytes: Transcriptomic, Proteomic, Morphological, and Functional Evidence. Neuron. 95:531
PMID: 28712653
Robinson and Wyatt (2015) Identification and structure solution of fragment hits against kinetoplastid N-myristoyltransferase. Nature. 71:586
PMID: 25945713
Xia et al. (2014) Regulation of action potential waveforms by axonal GABAA receptors in cortical pyramidal neurons. PLoS One. 9:e100968
PMID: 24971996
Cunningham et al. (2014) hPSC-derived maturing GABAergic interneurons ameliorate seizures and abnormal behavior in epileptic mice. Cell Stem Cell. 15:559
PMID: 25517465
Dettmann et al. (2014) Isolation, expansion and transplantation of postnatal murine progenitor cells of the enteric nervous system. PLoS One. 9:e97792
PMID: 24871092
Tsai et al. (2012) Autistic-like behaviour and cerebellar dysfunction in Purkinje cell Tsc1 mutant mice. MBio. 488:647
PMID: 22763451
Jerome et al. (2011) Parallel optical control of spatiotemporal neuronal spike activity using high-speed digital light processing. Front Syst Neurosci. 5:70
PMID: 21904526
Lam et al. (2011) Leptin does not directly affect CNS serotonin neurons to influence appetite. Acta Crystallogr F Struct Biol Commun. 13:584
PMID: 21531340

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