Biocytin

Name: Biocytin
Brand: Tocris Bioscience
SKU: 3349

Tocris Bioscience, part of Bio-Techne | Catalog # 3349

Citations (89)

COA / SDS

Versatile marker for neuroanatomical investigations

Key Product Details

Description:	Versatile marker for neuroanatomical investigations
Chemical Name:	N⁶-[5-[(3aS,4S,6aR)-Hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]-L-lysine
Molecular Weight:	372.48

View Full Technical Details for Biocytin

Technical Data
Citations

Biological Activity for Biocytin

Biocytin is a versatile marker used in anterograde, retrograde and intracellular neuroanatomical investigations and in biotinidase assays. Displays high solubility in aqueous solutions and has a low molecular weight facilitating injection using micropipettes. Can be incorporated with a variety of avidin and streptavidin conjugates for detection by light, fluorescence or electron microscope.

Technical Data for Biocytin

M. Wt	372.48
Formula	C₁₆H₂₈N₄O₄S
Storage	Store at -20°C
CAS Number	576-19-2
PubChem ID	83814
InChi Key	BAQMYDQNMFBZNA-MNXVOIDGSA-N
Smiles	O=C1N[C@@]2([H])[C@@](CS[C@H]2CCCCC(NCCCC[C@H](N)C(O)=O)=O)([H])N1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Biocytin

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	water	18.62	50 with gentle warming

Preparing Stock Solutions for Biocytin

The following data is based on the product molecular weight 372.48.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	5.37 mL	26.85 mL	53.69 mL
2.5 mM	1.07 mL	5.37 mL	10.74 mL
5 mM	0.54 mL	2.68 mL	5.37 mL
25 mM	0.11 mL	0.54 mL	1.07 mL

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Reconstitution Calculator

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References for Biocytin

References are publications that support the biological activity of the product.

Roth Morphology and axonal projection pattern of neurons in the telencephalon of the fire-bellied toad Bombina orientalis: an anterograde, retrograde and intracellular biocytin labelling study. J.Comp.Neurol. 2004 PMID: 15334648
MacNeil and Gaul Biocytin wide-field bipolar cells in rabbit retina selectively contact blue cones. J.Comp.Neurol. 2008 PMID: 17990268
Kobbert Current concepts in neuroanatomical tracing. Prog.Neurobiol. 2000 PMID: 10856608