Avidin D [Alkaline Phosphatase]
Novus Biologicals, part of Bio-Techne | Catalog # A-2100-NB
Key Product Details
Applications
ELISA, Functional Assay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Conjugate
Alkaline Phosphatase
Product Summary for Avidin D [Alkaline Phosphatase]
Features:
Suitable for use in solid-phase assays, tissue/cell staining systems, and blotting applications. Specific covalent linkages are chosen to provide stable, highly active conjugates. One unit is 1 µmole p-nitrophenylphosphate hydrolyzed per min. at 37 °C, pH 9.8.
Suitable for use in solid-phase assays, tissue/cell staining systems, and blotting applications. Specific covalent linkages are chosen to provide stable, highly active conjugates. One unit is 1 µmole p-nitrophenylphosphate hydrolyzed per min. at 37 °C, pH 9.8.
Product Specifications
Application Notes
To avoid adsorption or denaturing of the enzyme conjugate, dilutions may be made in 20 mM HEPES, 0.5 M NaCl, pH 7.5, containing 0.1 mg/mL IHC- grade BSA. DO NOT USE FRACTION V GRADE BSA. One unit equals 1.0 micromole of p-nitrophenol liberated from p-nitrophenylphosphate per minute at 37C (pH 9.5).
Recommended concentration for use is 0.2 units/mL.
Storage of dilute solutions of the enzyme conjugate is not recommended.
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Recommended concentration for use is 0.2 units/mL.
Storage of dilute solutions of the enzyme conjugate is not recommended.
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Scientific Data Images for Avidin D [Alkaline Phosphatase]
Avidin D [Alkaline Phosphatase] [A-2100-NB] - Alkaline Phosphatase Avidin D
Formulation, Preparation, and Storage
Preparation Method
Reconstitute with 1 mL water. The resulting solution will have the following composition: 10 mM HEPES, 0.15 M NaCl, 0.08% sodium azide, 1 mM Mg++, 0.1 mM Zn++, pH 7.5. This product has been stabilized by addition of Ficoll and IHC-grade BSA.
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at 4C. Do not freeze.
Background: Avidin D
Additional Avidin D Products
Product Documents for Avidin D [Alkaline Phosphatase]
Product Specific Notices for Avidin D [Alkaline Phosphatase]
This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
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